Immunoprecipitation of STING/ADSL and mass spectrometry analysis

ABSTRACT: BT-549 cells with or without expression of Flag-STING were treated with or without hypoxia for 6 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-STING were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Brilliant Blue. Selected peptide hits of proteins associated with Flag-STING identified through mass spectrometry are shown.Bacterially purified His-ADSL proteins on Ni-NTA agarose beads were incubated with or without active GST-IKKβ in the presence of ATP for an in vitro kinase assay;Then the protein samples were digested using GluC and analyzed using LC-MS/MS with the Orbitrap Elite mass spectrometer.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: yuran duan  

LAB HEAD: Daqian Xu

PROVIDER: PXD059420 | Pride | 2025-02-15

REPOSITORIES: Pride