Dataset Information

Clonorchis sinensis-Excretory/Secretory Proteins ameliorates Inflammation in Rheumatoid Arthritis and Ankylosing Spondylitis

ABSTRACT: We aimed to investigate whether substances secreted by Clonorchis sinensis-Excretory/Secretory protein (CS-ESP), rather than direct contact with crude proteins, have an effect on the inflammation of rheumatoid arthritis (RA) and ankylosing spondylitis (AS), and to identify specific peptides through related proteomic analysis to determine which proteins exhibit anti-inflammatory effects more specifically.

INSTRUMENT(S):

ORGANISM(S): Clonorchis Sinensis

SUBMITTER: Hee Min Yoo

LAB HEAD: Hee Min Yoo

PROVIDER: PXD059467 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CS_ESP.mzML	Mzml
	CS_ESP.xlsx	Xlsx

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Publications

Clonorchis sinensis excretory/secretory proteins ameliorate inflammation in rheumatoid arthritis and ankylosing spondylitis.

Kim Moon-Ju MJ Yoo Hee Min HM Lee Yu Jeong YJ Jang Hyun Hee HH Shim Seung Cheol SC Won Eun Jeong EJ Kim Tae-Jong TJ

Parasites & vectors 20250304 1

<h4>Background</h4>We aimed to investigate whether substances secreted by Clonorchis sinensis excretory/secretory protein (CS-ESP) have an effect on the inflammation of rheumatoid arthritis (RA) and ankylosing spondylitis (AS) and to identify specific peptides through related proteomic analysis to determine which proteins exhibit anti-inflammatory effects more specifically.<h4>Methods</h4>Peripheral blood mononuclear cells (PBMCs) were obtained from healthy controls (HCs), RA and AS patients. Cy ...[more]

PMID: 40038824

Similar Datasets

Project description:We have compared synovial biopsies from ankylosing spondylitis and undifferentiated spondylitis patients with healthy controls and osteoarthritis patients Objective: In spondylarthropies, whole-genome gene expression profiling studies have been limited to peripheral blood to date. By undertaking a study in knee synovial biopsies from spondylarthropy (SpA) and ankylosing spondylitis (AS) patients we aimed to identified joint-specific candidate genes and pathways. These pathways may mediate systemic inflammation driven joint damaging processes and more specifically, the osteoproliferation that is characteristic of these conditions. Methods: RNA was extracted from six seronegative SpA, two AS, three osteoarthritis (OA) and four normal control knee synovial biopsies. Whole genome expression profiling was undertaken using the Illumina DASL system, which assays 24000 cDNA probes. Differentially expressed candidate genes were then validated using quantitative PCR and immunohistochemistry. Results: 416 differentially expressed genes were identified that clearly delineated between AS/SpA and control groups. Pathway analysis showed altered gene-expression in oxidoreductase activity, osteoblast activity, B-cell associated, matrix catabolic, and metabolic pathways. The inflammatory mediator, MMP3, was strongly upregulated in AS/SpA samples and the Wnt pathway inhibitors DKK3 and Kremen1 were downregulated. Conclusion: Pathways mediating both systemic inflammation as well as local tissue changes were identified. This suggests initial systemic inflammation in spondylarthropies transfers to and persists in the local joint environment, subsequently mediating changes in genes directly involved in the destructive tissue remodelling. Fifteen knee synovial biopsy tissue samples consisting of six seronegative spondyloarthropy (SpA), two ankylosing spondylitis (AS), three osteoarthritis (OA) and four normal control biopsies were obtained from the Synovial Tissue Bank at the Repatriation General Hospital in Adelaide, South Australia with the appropriate ethical approval (Supplementary Table 1). All patients provided informed written consent.