Dataset Information

Combined cysteine oxidation (C6-CPT/CysPAT) proteomics and phosphoproteomics of pervanadate-treated of HEK293T cells

ABSTRACT: HEK293T cells were treated with pervanadate for 0, 5 or 15 minutes to evaluate the effects on the phosphorylation and cysteine oxidation status across the proteome. Samples were labelled withTMT 18plex and C6-CPT/CysPAT labelling was used to allow the simultaneous enrichment of both the phosphoproteome and total vs. oxidised cysteine proteome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Harvey Johnston

LAB HEAD: Hayley Sharpe

PROVIDER: PXD059503 | Pride | 2026-06-22

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	241024_CysPAT_Per15_eclipse.msf	Msf
	241024_CysPAT_Per15_eclipse.pdResult	Other
	241024_CysPAT_Per15_eclipse_OxC.msf	Msf
	241024_CysPAT_Per15_eclipse_OxC.pdResult	Other
	241024_CysPAT_Per15_eclipse_input.msf	Msf

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Publications

Pervanadate-induced oxidation relieves autoinhibition of the protein tyrosine kinase SRC.

Mulholland Katie E KE Bourguet Maxime M Cheng Nuo N Rahman Oisharja O Ezeriņa Daria D Daly Leonard A LA Lai Tiffany T Aldaz Casanova Silvia S Featherston Therese T Creixell Pau P Eyers Claire E CE Messens Joris J Eyers Patrick A PA Byrne Dominic P DP Sharpe Hayley J HJ

Science signaling 20260120 921

Dynamic regulation of protein tyrosine phosphorylation (pTyr) by kinases and phosphatases enables cells to sense and respond to environmental changes. The widely used chemical pervanadate induces the accumulation of pTyr in mammalian cell lines. This effect is primarily attributed to its inhibition of protein tyrosine phosphatases (PTPs), leading to the assertion that PTPs are master gatekeepers of intracellular pTyr homeostasis. Here, we used several approaches to reveal that pervanadate disrup ...[more]

PMID: 41557758

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