Proteomics of Small Extracellular Vesicles from Melanoma DCC-Derived Cell Lines

ABSTRACT: Small extracellular vesicles (sEVs) were isolated from the culture supernatant of melanoma DCC-derived cell lines (MelDCC) after 48 hours of serum-free culture. sEVs were obtained through differential ultracentrifugation, specifically as the 100K pellet, following the protocol described in detail by Théry et al. (https://doi.org/10.1002/0471143030.cb0322s30). Proteomic analysis of the isolated sEVs was performed using mass spectrometry to characterize their protein content.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Peter Oefner  

LAB HEAD: Peter J. Oefner

PROVIDER: PXD059510 | Pride | 2025-02-24

REPOSITORIES: Pride