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Probing the trypanosome nuclear pore by TurboID proximity labelling – Part3

ABSTRACT: Previous BioID experiments targeting the nucleoporin (NUP) NUP158 (PMID: 36410438; PXD031245), as well as NUP110, NUP76, NUP96 (PMID: 39206942; PXD047268)and NUP75, transport factors MEX67 and Ran(PXD055934), indicated that proximity labelling delivers highly specific interactome data in the confined localisation of the nuclear pore, with a labelling radius well below the size of the nuclear pore. The resulting proximity data allowed to assign NUPs and nuclear transport factors to specific subregions of the pore. Here we extended this approach to target NUP98.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Trypanosomiasis

SUBMITTER: Martin Zoltner

LAB HEAD: Martin Zoltner

PROVIDER: PXD059554 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	NUP98N_1.raw	Raw
	NUP98N_2.raw	Raw
	NUP98N_combined.txt.zip	Txt
	TriTrypDB-64_TbruceiTREU927_AnnotatedProteins.fasta	Fasta

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Probing the trypanosome nuclear pore by TurboID proximity labelling – Part2

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