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Proteomic analysis of bronchial epithelium challenged with bronchial smooth muscle extracellular vesicles

ABSTRACT: The main objective of CHARMACOMA is to explore the capacity of the severe asthmatic bronchial smooth muscle to modify bronchial epithelium physiology from a metabolic point of view.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bronchial Epithelial Cell

SUBMITTER: Dupuy Jean-William

LAB HEAD: Pauline Esteves

PROVIDER: PXD059603 | Pride | 2026-02-09

REPOSITORIES: Pride

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			Action	DRS
	Link_between_files.xlsx	Xlsx
	RP_UP000005640_Homo_sapiens_2023_09_13.fasta	Fasta
	checksum.txt	Txt
	ep241219_01b.msf	Msf
	ep241219_01b.pdResult	Other

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Publications

Bronchial smooth muscle extracellular vesicles interfere with bronchial epithelium metabolism and function in asthma.

Celle Elisa E Chahin Amine A Beaufils Fabien F Cardouat Guillaume G Eyraud Edmée E Bouchet Clément C Campagnac Marilyne M Ousova Olga O Begueret Hugues H Thumerel Matthieu M Dubois Rémi R Dupuy Jean-William JW Leste-Lasserre Thierry T Lacomme Sabrina S Lager-Lachaud Nina N Bellvert Floriant F Marthan Roger R Girodet Pierre-Olivier PO Berger Patrick P Trian Thomas T Esteves Pauline P

iScience 20250429 6

Bronchial smooth muscle (BSM) remodeling is an important feature of severe asthma pathophysiology. We previously showed that asthmatic BSM is metabolically different and increased rhinovirus (RV) replication rate, the main trigger of severe asthma exacerbations. Extracellular vesicles (EVs) are the key mediator in cell-cell communication, but the role of BSM cells-derived EVs on bronchial epithelial has never been investigated in asthma. Using severe asthmatic and non-asthmatic tissue collection ...[more]

PMID: 40487443

Similar Datasets

Proteomic analysis of bronchial epithelium challenged with bronchial smooth muscle extracellular vesicles

Project description:The main objective of CHARMACOMA is to explore the capacity of the severe asthmatic bronchial smooth muscle to modify bronchial epithelium physiology from a metabolic point of view.

2026-02-09 | PXD055949 | Pride

Characterization of a new variant of SERPINA1 Gene Causing Alpha-1 Antitrypsin Deficiency

Project description:Alpha 1-Antitrypsin Deficiency (AATD) is a rare genetic disorder caused by mutations in the SERPINA1 gene, which encodes the Alpha 1-Antitrypsin (AAT) protein. Individuals carrying pathogenic SERPINA1 variants are predisposed to lung and liver damage. The most common AATD-associated SERPINA1 alleles are the S and Z alleles, though additional variants have been identified. This case report describes the discovery of a new SERPINA1 variant detected in two cities in southeastern France. This variant, named PiZmarseille, results from in cis combination of the PiZ allele and a rare variant known as PiZbristol. PiZmarseille has been associated with early-onset liver disease in childhood. To further investigate this new variant, we performed its molecular characterization, revealing that PiZmarseille shares the pathogenic properties of both the PiZ and PiZbristol variants. These properties include its retention in the endoplasmic reticulum as aggregates and its degradation through the autophagy and proteasome pathways. Additionally, we conducted proteomic profiling to explore the association of this mutant with liver disease. Our analysis revealed that the neutrophil degranulation pathway is particularly deregulated in PiZmarseille liver samples. Furthermore, when compared to other AAT genotypes, the proteomic profile of PiZmarseille most closely resembles that of the PiZ variant, rather than other SERPINA1 variants.

2025-12-08 | PXD060960 | Pride

Proteomic analysis of bronchial smooth muscle cells extracellular vesicles

2025-11-26 | PXD055968 | Pride

Severe asthmatic bronchial smooth muscle decreases epithelial response to rhinovirus infection.

Project description:Asthma is a very frequent airway disease that affects 6 to 20% of the population. Severe asthma, represents 3 to 5% of all asthmatic patients and is histologically characterized by an increased bronchial smooth muscle (BSM) mass and clinically by viral exacerbations. Functionally, BSM remodeling had a poor prognostic value in asthma, since higher BSM mass was associated with lower lung function and increased exacerbation rate. However, the role of BSM as a potential actor of asthma exacerbation has only been sparsely suggested. We thus hypothesis that asthmatic BSM cells could act on bronchial epithelium and modified its response to rhinovirus infection.

2023-05-02 | PXD015604 | Pride

The crucial role of CPT2 in the asthmatic bronchial smooth muscle remodeling

Project description:Asthma is a very frequent airway disease that affects 6 to 20% of the population. Severe asthma, represents 3 to 5% of all asthmatic patients and is histologically characterized by an increased bronchial smooth muscle (BSM) mass and clinically by viral exacerbations. Functionally, BSM remodeling had a poor prognostic value in asthma, since higher BSM mass was associated with lower lung function and increased exacerbation rate. However, the role of BSM as a potential actor of asthma exacerbation has only been sparsely suggested. Thus, we hypothesis that asthmatic BSM cell metabolism is modified compare to that of non-asthmatic and that could be a potential target to reduce asthmatic BSM cell proliferation and remodeling in asthma.

2021-06-10 | PXD015566 | Pride

microRNA expression data in bronchial epithelial cells from healthy and asthmatic donors

Project description:Airway inflammation is the hallmark of asthma and suggests a dysregulation of homeostatic mechanisms. MicroRNAs (miRNAs) are key regulators of gene expression, necessary for the proper function of cellular processes. We used miRNA microarrays to compare the profiles of human bronchial epithelial cells from healthy and asthmatic donors Human bronchial epithelial cells were isolated from healthy and asthmatic donors. RNA was extracted and miRNA expression was analyzed on Affymetrix miRNA microarrays. We sought to utilize miRNA expression as a tool for understanding underlying biological differences in BECs from healthy and asthmatic donors.

2012-10-31 | E-GEOD-25230 | biostudies-arrayexpress

The effect of apolipoprotein E on asthmatic bronchial brushing cell gene signature

Project description:Human asthmatic bronchial brushing cells stimulated with apolipoprotein E demonstrate increased expression of mRNA transcripts encoding multiple pro-inflammatory genes

2020-03-01 | GSE128497 | GEO

Expression data from human bronchial airway smooth muscle (ASM) cells

Project description:Interleukin (IL)-17 plays an important and protective role in host defence and has been demonstrated to orchestrate airway inflammation by cooperating with and inducing proinflammatory cytokines. Mircoarrays were used to identify immediate-early/ primary response IL-17A-dependent gene transcripts in primary human bronchial ASM cells from mild asthmatic and healthy individuals. To evaluate IL-17A-inducible gene transcripts, primary human bronchial ASM cells from 3 mild asthmatic and 3 healthy donors were treated for 2h with IL-17 [10ng/ml] and were probed with the Affymetrix GeneChip array. The 2h time point was carefully chosen in order to identify primary response gene targets and to avoid confounding autocrine mechanisms mediating indirect, or late-phase gene expression responses. Non-stimulated ASM cells from the same patients were used as controls.

2012-02-09 | E-GEOD-35643 | biostudies-arrayexpress

DNA methylation in airway epithelial cells isloated by pronase and bronchial brush from individuals with and without asthma

Project description:The airway epithelium forms the interface between the inhaled environment and the lung. The airway epithelium is dysfunctional in asthma and epigenetic mechanisms are considered a contributory factor. We hypothesised that the DNA methylation profiles of cultured primary airway epithelial cells (AECs) would differ between cells isolated from individuals with asthma (n=17) versus those without asthma (n=16). AECs were isolated from patients by two different isolation techniques; pronase digestion (9 non-asthmatic, 8 asthmatic) and bronchial brushings (7 non-asthmatic and 9 asthmatic). DNA methylation was assessed using an Illumina Infinium HumanMethylation450 BeadChip array. DNA methylation of AECs clustered by isolation technique and linear regression identified 111 CpG sites differentially methylated between isolation techniques in healthy individuals. As a consequence, the effect of asthmatic status on DNA methylation was assessed within AEC samples isolated using the same technique. In pronase isolated AECs, 15 DNA regions were differentially methylated between asthmatics and non-asthmatics. In bronchial brush isolated AECs, 849 differentially methylated DNA regions were identified with no overlap to pronase regions. In conclusion, regardless of cell isolation technique, differential DNA methylation was associated with asthmatic status in AECs, providing further evidence for aberrant DNA methylation as a signature of epithelial dysfunction in asthma.

2019-10-31 | GSE137716 | GEO

Expression data from human bronchial airway smooth muscle (ASM) cells

2012-02-09 | GSE35643 | GEO