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Deciphering the proteomic profile of the parasite Fasciola hepatica during host migration reveals new insights into early-stage infection

ABSTRACT: The project presents an in vivo mouse model coupled with quantitative SWATH-MS proteomics to study the early stages of Fasciola hepatica migration, focusing on the parasite's passage through the intestinal barrier to peritoneum(24 hours post-infection) and migration to the liver parenchyma (8 days post-infection). This approach aims to assess the proteomic changes in the parasite during these critical stages of infection.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Fasciola Hepatica (liver Fluke)

SUBMITTER: Marta López-García

LAB HEAD: Mar Siles-Lucas

PROVIDER: PXD059684 | Pride | 2025-06-05

REPOSITORIES: Pride

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			Action	DRS
	Fh24h_S1.wiff	Wiff
	Fh24h_S1.wiff.scan	Wiff
	Fh24h_S2.wiff	Wiff
	Fh24h_S2.wiff.scan	Wiff
	Fh24h_S3.wiff	Wiff

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Publications

Deciphering the proteomic profile of the parasite Fasciola hepatica after initial vertebrate host infection reveals new insights into its early-stages.

López-García Marta M Cwiklinski Krystyna K Becerro-Recio David D Ruiz-Campillo María Teresa MT Molina-Hernández Verónica V Pérez-Arévalo José J Martínez-Moreno Álvaro Á González-Miguel Javier J Siles-Lucas Mar M

Molecular & cellular proteomics : MCP 20250530

The migration of the trematode parasite Fasciola hepatica within its vertebrate host following infection by ingestion of the metacercariae represents a critical event in its establishment and survival. The early stages of infection, during which F. hepatica crosses the intestinal barrier and advances to the liver through the peritoneum, initiate changes in the parasite that drive its development from a free-living state on pasture to an obligate blood-feeding parasite. Using an in vivo mouse mod ...[more]

PMID: 40451427

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Project description:Understanding how P4 signaling occurs in endometrial cells is important to guide the development of more effective therapeutic approaches to the numerous reproductive diseases caused by disrupted P4 signaling, resulting in subfertility or infertility (Bunch et al., 2013; Kim et al., 2013; Zhang and Murphy, 2014; Szekeres-Bartho, 2018; Hirota, 2019; Liao et al., 2019). Here, to fill these knowledge gaps, we characterized endometrial PGRMC2 expression throughout the human menstrual cycle and in hEnSCs subjected to an in vitro model of human endometrial decidualization. Additionally, we describe the functional implications of PGRMC2 with a silencing approach assay, integrating RNA-seq and proteomic techniques. We evaluated the functional implication of PGRMC2 in the embryo implantation process with an outgrowth in vitro model. Finally, we also compared endometrial PGRMC2 expression with PGRMC1 and PGR.

2024-06-22 | PXD048494 | Pride

Translation factor eIF5A controls mitochondrial protein import through the translation regulation of the mitochondrial inner membrane translocase receptor Tim50

Project description:The import of nuclear-encoded proteins into mitochondria is a key process for the right mitochondrial function. The evolutionarily conserved translation factor eIF5A is primarily known as an elongation factor which binds ribosomes to facilitate the translation of motifs with polyprolines or combinations of proline with glycine and charged amino acids. eIF5A expression is up-regulated by the transcription factor Hap1 under respiratory conditions and is essential to maintain the mitochondrial activity. Here we identify an essential polyproline-containing protein as a direct eIF5A target for translation: the mitochondrial inner membrane protein Tim50, which is the receptor subunit of the TIM23 translocase complex. We show how eIF5A directly controls the mitochondrial protein import through the alleviation of ribosome stalling along Tim50 sequence at the mitochondrial surface. Thus, the absence of eIF5A leads to the accumulation of mitochondrial protein precursors in the cytosol as well as to the induction of the mitoCPR stress response. Moreover, we show that eIF5A also affects mitochondrial functionality by reducing the translation and levels of many mitochondrial proteins. Overall, these results show for the first time how ribosome stalling negatively impacts the mitochondrial import process and explain why eIF5A is essential for the mitochondrial function.

2024-10-23 | PXD043905 | Pride

Proteomic variation in the oral secretion of Spodoptera exigua and Spodoptera littoralis larvae in response to different food sources

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2025-01-23 | PXD056472 | Pride

Effect of capacitation on proteomic profile and mitochondrial parameters of spermatozoa in bulls

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2025-05-07 | PXD056424 | Pride

2. Proteomic Insights into Seminal Plasma and Spermatozoa of Small-Spotted Catsharks (Scyliorhinus canicula): Differential expression analysis

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2024-05-22 | PXD046348 | Pride

Proteomic and pathway profiling of four Breast Cancer cell lines.

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2023-09-07 | PXD043759 | Pride

Proteotranscriptomic analysis of advanced colorectal cancer patient derived organoids for drug sensitivity prediction

Project description:Understanding the phenotypic alterations that play a role in differential response to drugs will be pivotal in identifying predictive biomarkers and developing efficacious therapies for advanced CRC. The bulk of research to date has focused on the value of organoids as a predictive tool; some have characterized the transcriptomic profile in relation to drug sensitivity in an effort to identify predictive biomarkers, but only a limited number of studies have used quantitative proteomic analysis to characterize PDOs. Extensive literature indicates that transcriptomic and proteomic expression profiles lack correlation due to significant post-transcriptional regulation, which could explain why RNA signatures are rarely useful as drug response predictors in the clinic. In this study we generated PDO models from advanced CRC patients including heavily pre-treated patients and a model of microsatellite instable- (MSI) high CRC, for which pre- and post chemotherapy liver disease PDOs were established and characterized to ensure biological fidelity with the original tumors. Our main objective was to integrate functional drug assays on PDOs with proteotranscriptomic study to explore the mechanisms underlying drug response. To this end, we used SWATH-MS (sequential window acquisition of all theoretical mass spectrometry), a highly accurate proteomic quantification technique and a bulk RNA-seq analysis. This strategy could represent a useful tool to discover new therapeutic targets in advanced CRC and to guide personalized therapies.

2025-02-03 | PXD038149 | Pride

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Project description:The global imperative to enhance crop protection while preserving the environment has increased interest in the application of biological pesticides. Bacillus thuringiensis (Bt) is a Gramm-positive bacterium that can produce nematicidal proteins and accumulate them in parasporal crystals. Root-knot nematodes are obligate root plant parasitic which are distributed worldwide, causing severe damages to the infested plants and, consequently, large yield reductions. In this work, we have evaluated the toxicity of the crystal proteins Cry5, Cry21, App6, and Xpp55 against two root-knot nematodes belonging to the Meloidogyne genus (M. incognita and M. javanica). The results show that all four proteins, when solubilized, were highly toxic for both nematode species. To check the potential of using Bt strains producing nematicidal crystal proteins as biopesticides to control plant parasitic nematodes in the field, in planta assays were conducted, using two wild Bt strains which produced Cry5 or a combination of App6 and Cry5 proteins. The tests were carried out with cucumber or with tomato plants infested with M. javanica J2, subjected to irrigation with spore+cristal mixtures of the respective strains. The results showed that the efficacy of the nematicidal activity was plant-dependent, as Bt was able to reduce emerged J2 in tomato plants but not in cucumber plants. In addition, the toxicity observed in the in planta assays was much lower than expected, highlighting the challenge of the crystal proteins to exert their toxicity. This emphasizes the delivery of the Bt proteins as crucial for its use to control root-knot nematodes.

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Post-transcriptional control of a stemness signature by RNA-binding 8 protein MEX3A regulates adult neurogenesis

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