Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Micropillar-induced changes in cell nucleus morphology enhance bone regeneration by modulating the secretome

ABSTRACT: To investigate the influence of micropillars on proteomics, we seeded cells on flat and micropillar surfaces followed by (1) collection of proteins in cells on flat and micropillar surfaces; (2) collection of proteins secreted into medium by cells on flat and micropillar surfaces.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Dr. Indira Pla Parada

LAB HEAD: Guillermo A. Ameer

PROVIDER: PXD059752 | Pride | 2025-05-19

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20230815_yru5928_23-3199_Ameer_Wang_10.raw	Raw
	20230815_yru5928_23-3199_Ameer_Wang_11.raw	Raw
	20230815_yru5928_23-3199_Ameer_Wang_12.raw	Raw
	20230815_yru5928_23-3199_Ameer_Wang_7.raw	Raw
	20230815_yru5928_23-3199_Ameer_Wang_8.raw	Raw

Items per page:

1 - 5 of 16

Similar Datasets

Investigation of titanium coated imprinted polystyrene surfaces

Project description:In this study the gene expression differences between two titanium surfaces produced at Maastricht University were investigated. These two surfaces were: flat titanium-coated polystyrene and a titanium-coated polystyrene surface imprinted with a pattern selected from an earlier screening study (Ti1018). This pattern was selected based on the osteoinductive properties observed. As a positive control cells on the flat surface were treated with dexamethasone.

2018-09-25 | E-MTAB-7212 | biostudies-arrayexpress

Investigation of titanium discs produced by Straumann on human mesenchymal stromal cells

Project description:In this study the gene expression differences between three titanium surfaces produced by Straumann were investigated. These three surfaces were: flat pre-treated (Pt) titanium, sandblasted (S) titanium and sandblasted acid-etched (SLA) titanium. The SLA surface is known to boost the proliferation and osteogenic differentiation of MG-63 cells. SLA titanium is also widely used for dental implants.

2018-09-05 | E-MTAB-7217 | biostudies-arrayexpress

Nanotopography guides morphology and spatial patterning of induced pluripotent stem cell colonies

Project description:The role of topographic cues in controlling commitment of induced pluripotent stem cells (iPSCs) is largely unknown. Here we demonstrate that groove-ridge nanostructures induce the elongation of iPSC colonies, guide the orientation of apical actin fibers and direct the polarity of cell division. Elongation of iPSC colonies impacts also on the intrinsic molecular patterning which seems to be orchestrated starting from the rim of the colonies. We followed the hypothesis that nanotopography directly modulates the transcriptional program of iPSC, further to guiding the overall spatial organization of the colonies. Single iPSC were seeded on flat (PI flat) and nanostructured polyimide (PI 650) and gene expression profiles were analyzed after three days. No significant differences were observed when cells were kept under culture conditions that sustained pluripotency. Then, we analyzed gene expression changes upon two weeks of multi-lineage differentiation. Many genes revealed significant expression changes in the course of differentiation and this was more pronounced on PI flat as compared to PI 650. Comparison of iPSC that were either differentiated on flat or nanostructured biomaterials revealed differential expression of several genes. Noteworthy, among significantly regulated genes, the biggest fold change on PI 650 versus PI flat after differentiation was observed in ANKRD1, which is one of the best readouts of YAP/TAZ activity. Our study suggests that nanotopography impacts on orientation and organization of iPSC colonies and highlight a possible interaction between mechanosensors and mechanotransducers.

2017-09-18 | GSE84848 | GEO

Microtopography-induced changes in cell nucleus morphology enhance bone regeneration by modulating the cellular secretome

Project description:To investigate the influence of micropillar implants on bone regeneration, we created two critical size cranial wound repair model using implants with flat and micropillar surface topography

2025-05-01 | GSE286676 | GEO

Effects of carbazole-degradative plasmid pCAR1 on biofilm morphology in three Pseudomonas strains.

Project description:To elucidate the impact of the carriage of carbazole-degradative plasmid pCAR1 on biofilm formation by host bacteria, we compared biofilm morphology of pCAR1-free and pCAR1-carrying three Pseudomonas hosts: P. putida KT2440, P. aeruginosa PAO1 and P. fluorescens Pf0-1 using confocal laser scanning microscopy. Although pCAR1 carriage had no significant influence on biofilm formed by PAO1 and Pf0-1, pCAR1-carrying KT2440 became filamentous and formed flat biofilm, whereas pCAR1-free KT2440 formed mushroom-like biofilm. pCAR1 carries three genes encoding nucleoid-associated proteins (NAPs); Pmr, Pnd, and Phu. Because intensive filamentous morphology was observed in two double mutants (DpmrDpnd and DpmrDphu), these NAPs cooperatively suppress cell filamentation and flat biofilm formation in KT2440(pCAR1) cells. Based on the results of transcriptome analyses of these double mutants, we could find 32 candidate genes which may be involved in the filamentation of KT2440(pCAR1). Overproduction in KT2440 and KT2440(pCAR1) of three of candidate genes (PP_2193, PP_0308, or PP_0309) induced temporal filamentation of the cells, suggesting that pCAR1 induced the abnormal filamentous morphology of KT2440 cells by overexpression of plural genes including PP_2193, PP_0308, and PP_0309. In addition, pCAR1-borne NAPs suppress the morphological changes probably by decreasing the transcriptome change by pCAR1 carriage. The pCAR1 carriage impact of chromosomal RNA maps in biofilm formation.

2014-05-08 | E-GEOD-56856 | biostudies-arrayexpress

Effects of carbazole-degradative plasmid pCAR1 on biofilm morphology in three Pseudomonas strains.

2014-05-08 | GSE56856 | GEO

Flat Dose vs. Weight-based IP Chemotherapy for CRS/HIPEC

Project description:Peritoneal carcinomatosis from advanced gastro-intestinal malignancy has historically been associated with poor overall survival (≤ 12 months) with few treatment options. Cytoreductive surgery (CRS), which involves removal of all macroscopic tumor nodules, combined with direct administration of heated intra-peritoneal (IP) chemotherapy (HIPEC) to the affected peritoneal surfaces, has been shown to be an effective treatment option that extends overall survival among certain cases of peritoneal carcinomatosis. IP chemotherapy allows delivery of a high dose of cytostatic drug directly onto the peritoneal surfaces at risk for microscopic residual disease while systemic exposure remains limited. Additionally, hyperthermia is known to enhance the cytotoxicity of several agents (including Mitomycin C) and improves the depth of peritoneal penetration. This trial will be a randomized phase 2 comparison of flat dose versus weight-based dose Mitomycin C. The hypothesis of this study is that HIPEC weight-based dosing may result in similarly effective peritoneal Mitomycin C concentrations with less systemic absorption and potential systemic toxicity, compared with the HIPEC flat dosing approach in patients undergoing CRS/HIPEC.

| 2369290 | ecrin-mdr-crc

Expression data of mRNA from normal utricle and vestibular flat epithelium of adult mice

Project description:The vestibular sensory epithelium may degenerate into a layer of flat cells, known as flat epithelium, after a severe lesion, but the pathogenesis of vestibular flat epithelium remains unclear. We used microarrays to detail the global programme of gene expression in normal utricle and vestibular flat epithelium and identified whether epithelial-mesenchymal transition participite in this process

2021-09-30 | GSE179063 | GEO

DNA methylation data from human lymphoblasts [Illumina]

Project description:DNA methylation profiling of IAS subjects Lymphoblasts RNA isolated from Iowa Adoption Study participants to compare the relationship between DNA methylation and gene expression on a genome-wide scale

2012-12-28 | E-GEOD-34777 | biostudies-arrayexpress