Dataset Information

Polystyrene micro- and nanoplastics aggravates colitis in a mouse model – effects on biodistribution, macrophage polarization, and gut microbiome

ABSTRACT: The increasing prevalence of inflammatory bowel disease (IBD) and rising pollution from micro- and nanoplastic (MNP) particles has prompted investigations on their potential interconnection. To elucidate the complex relationship between IBD and exposure to MNPs, we induced colitis in mice using dextran sodium sulfate (DSS) and orally administered a solution of polystyrene (PS) MNPs (diameter 10, 1, and 0.29 µm). These particles enabled a detailed examination of MNP biodistribution, macrophage response, and gut microbiome alterations under inflammatory conditions. Specifically, the nanosized PS particles predominantly accumulated in the bloodstream and excretory organs, with enhanced accumulation in the colitis model. Proteomic analysis of the colon revealed alterations in molecular pathways related to protein transport, metabolism, and immune responses. Specifically, macrophages shifted towards a pro-inflammatory phenotype, highlighting the intricate effects of MNPs on inflammation and immune cell behavior. Moreover, MNPs significantly disrupted the gut microbiome, reducing microbial diversity and shifting bacterial populations towards pro-inflammatory and potentially pathogenic species. These changes suggest that MNP exposure could exacerbate colitis through complex interactions involving MNPs, immune responses, and microbial dynamics. The fast-growing exposure to MNPs underscores the urgent need for comprehensive strategies to address MNP pollution, its implications for disease, and potential impacts on public health

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon, Small Intestine

DISEASE(S): Colitis

SUBMITTER: Ulrike Resch

LAB HEAD: Ulrike Resch

PROVIDER: PXD059763 | Pride | 2026-05-22

REPOSITORIES: Pride

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			Action	DRS
	01_supplTable_1_final.zip	Other
	02_supplTable_2_final.zip	Other
	20220917_Q1_DIA60min_poolCo_DSS_G4.zip	Other
	20220917_Q1_DIA60min_poolCo_G2.zip	Other
	20220917_Q1_DIA60min_poolCo_MNPG6.zip	Other

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Polystyrene micro- and nanoplastics aggravates colitis in a mouse model – effects on biodistribution, macrophage polarization, and gut microbiome

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