Proteomics

Dataset Information

0

Identification of the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212)

ABSTRACT: Segmented filamentous bacteria (SFB) are ubiquitous intestinal commensals that promote immune system development and pathogen protection. The unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), attach to ileal epithelial cells via their unique tip structure and outgrow into filaments. To obtain a more detailed view of the SFB surface, particularly at the tip structure, we used a combination of cryo-EM and cryo-ET to characterize the surface structures present in IOs and filaments from mouse- and rat-SFB. HDX-MS was used to identified the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212) located at the SFB surface.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Sébastien Brier  

LAB HEAD: Sebastien BRIER

PROVIDER: PXD060041 | Pride | 2025-10-21

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20240710_S_SBr_AID45212_PepMap_High_01_IA_final_peptide.csv Csv
20240710_S_SBr_AID45212_PepMap_Low_02_IA_final_peptide.csv Csv
20240710_S_SBr_AID45212_PepMap_Med_01_IA_final_peptide.csv Csv
20240711_S_SBr_AID45212_PepMap_Mix_01_IA_final_peptide.csv Csv
FileDescription.xlsx Xlsx
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Publications

Segmented filamentous bacteria (SFB) are intestinal commensals that promote immune system development and pathogen protection through intimate attachment to the ileal epithelium. Attachment occurs via the tip of unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), before outgrowth into filaments. To characterize this critical stage of the SFB life cycle, we imaged SFB using cryo-electron microscopy and tomography. IOs were surrounded by a repetitive surface (S)-layer that beca  ...[more]

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