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Identification of the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212)

ABSTRACT: Segmented filamentous bacteria (SFB) are ubiquitous intestinal commensals that promote immune system development and pathogen protection. The unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), attach to ileal epithelial cells via their unique tip structure and outgrow into filaments. To obtain a more detailed view of the SFB surface, particularly at the tip structure, we used a combination of cryo-EM and cryo-ET to characterize the surface structures present in IOs and filaments from mouse- and rat-SFB. HDX-MS was used to identified the epitope recognized by a specific VHH raised against the Th17 antigen (AID45212) located at the SFB surface.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Sébastien Brier

LAB HEAD: Sebastien BRIER

PROVIDER: PXD060041 | Pride | 2025-10-21

REPOSITORIES: Pride

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			Action	DRS
	20240710_S_SBr_AID45212_PepMap_High_01_IA_final_peptide.csv	Csv
	20240710_S_SBr_AID45212_PepMap_Low_02_IA_final_peptide.csv	Csv
	20240710_S_SBr_AID45212_PepMap_Med_01_IA_final_peptide.csv	Csv
	20240711_S_SBr_AID45212_PepMap_Mix_01_IA_final_peptide.csv	Csv
	FileDescription.xlsx	Xlsx

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