Dataset Information

Proteomic Analysis of Receptive and Non-receptive Endometria-derived Uterine Fluid by LC-MS/MS

ABSTRACT: The current diagnostic tools used to determine the precise timing of embryo transfer for in vitro fertilization (IVF)-frozen embryo transfer (FET) patients are not non-invasive or accurate. As uterine fluid is collected via a minimally invasive procedure and reflects alterations in the molecular composition of the endometrium during the menstrual cycle, it can be considered an accurate diagnostic source for identifying endometrial receptivity-related proteins. Therefore, we aimed to identify a panel of molecular biomarkers for endometrial receptivity by investigating uterine fluid lavage (UFL). The UFLs of 18 IVF-FET patients were collected two days before FET. After seven weeks, the patients were divided into receptive and non-receptive groups on the basis of ultrasonography results for clinical pregnancy. Protein profiling of the collected UFLs was performed via tandem mass tag labeling and mass spectrometry liquid chromatography. Next, differentially abundant proteins between the two groups were identified. Additionally, the largest contributions of the variable to the differences based on the variable of importance in projection (VIP) score value were inspected. The candidate protein panel was selected on the basis of the VIP score and protein database analysis. The accuracy of the candidate panel was assessed via logistic regression as a machine learning approach. Finally, western blot analysis confirmed the results.

INSTRUMENT(S): Q-Tof Ultima, LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Uterine Fluid

SUBMITTER: Farnoosh Saraee

LAB HEAD: Ghasem Hosseini Salekdeh

PROVIDER: PXD060077 | Pride | 2025-06-02

REPOSITORIES: Pride

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			Action	DRS
	200710_P90068_IVF_TMT1_HpH_1.raw	Raw
	200710_P90068_IVF_TMT1_HpH_10.raw	Raw
	200710_P90068_IVF_TMT1_HpH_11.raw	Raw
	200710_P90068_IVF_TMT1_HpH_12.raw	Raw
	200710_P90068_IVF_TMT1_HpH_13.raw	Raw

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Project description:The aberrant gene expression in the uterine endometrium and embryo has been the major causes of pregnancy failure in cattle. Therefore, selecting cows having adequate endometrial receptivity and embryos of better developmental competence based on the gene expression could increase the number of calves produced by each cow during its productive life time. We used endometrial and embryo biopsy technology in conjunction with the pregnancy outcome information to establish a direct link between the pre-transfer endometrial or in vivo derived embryo gene expression and pregnancy outcome after embryo transfer. Endometrial samples were collected from Simmental heifers at day 7 and 14 of the estrous cycle, one cycle prior to embryo transfer. In the next cycle, embryo biopsies consisting of 60-70% of inner cell mass and trophectoderm were transferred to the recipients at day 7 of the estrous cycle. The remaining 30-40% parts of the embryos were retained for analysis.Pregnancy diagnosis was performed at days 28 and 42 by ultrasonography and at day 56 by rectal palpation. Those heifers returned to heat at day 21 were considered as non pregnant or non receptive endometrium (NP) while those heifers ended up with successful pregnancy and calf delivery was considered as the calf delivery group or receptive endometrium (CD). Following this, the endometrial samples collected during the pre-transfer period and the embryo biopsies retained during embryo transfer were categorized based on the pregnancy outcome. Those endometrial biopsies collected at days 7 and 14 of the estrous cycle from heifers resulted in successful calf delivery were designated as CDd7 and CDd14, respectively and endometrial biopsies taken at days 7 and 14 of the estrous cycle from those subsequently resulted in no pregnancy were designated as NPd7 and NPd14, respectively. Similarly, the embryo biopsies were classified as those embryo biopsies resulted in successful calf delivery and those resulted in no pregnancy

Dataset Information

Proteomic Analysis of Receptive and Non-receptive Endometria-derived Uterine Fluid by LC-MS/MS

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