Proteomics

Dataset Information

Chemical crosslinking of plastocyanin and the cytochrome b6f complex


ABSTRACT: In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well characterized, the interaction between PC and Cytf remains less clear. Using chemical crosslinking combined with mass spectrometry, we identified two potential binding models for PC and Cytf: Side-on and Head-on.

INSTRUMENT(S):

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Martin Scholz  

LAB HEAD: Michael Hippler

PROVIDER: PXD060078 | Pride | 2025-08-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
All_Crosslinks_ox-red.xlsx Xlsx
All_crosslinks_PC_mutants.xlsx Xlsx
DW_06062023_Gel_Cyt-PC_ox-ox.raw Raw
DW_06062023_Gel_Cyt-PC_ox-red.raw Raw
DW_06062023_Gel_Cyt-PC_red-ox.raw Raw
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