Dataset Information

Chemical crosslinking of plastocyanin and the cytochrome b6f complex

ABSTRACT: In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well characterized, the interaction between PC and Cytf remains less clear. Using chemical crosslinking combined with mass spectrometry, we identified two potential binding models for PC and Cytf: Side-on and Head-on.

INSTRUMENT(S):

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Martin Scholz

LAB HEAD: Michael Hippler

PROVIDER: PXD060078 | Pride | 2025-08-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	All_Crosslinks_ox-red.xlsx	Xlsx
	All_crosslinks_PC_mutants.xlsx	Xlsx
	DW_06062023_Gel_Cyt-PC_ox-ox.raw	Raw
	DW_06062023_Gel_Cyt-PC_ox-red.raw	Raw
	DW_06062023_Gel_Cyt-PC_red-ox.raw	Raw

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Publications

Insights into plastocyanin-cytochrome b6f complex formation: The role of plastocyanin phosphorylation.

Milrad Yuval Y Wegemann Daniel D Kuhlgert Sebastian S Scholz Martin M Younas Muhammad M Vidal-Meireles André A Hippler Michael M

Plant physiology 20250801 4

Plastocyanin (PC) is a copper-containing protein that acts as a mobile electron carrier in plants during photosynthesis. In this work, we investigated the role of PC phosphorylation in photosynthetic electron transfer, focusing on interactions with both cytochrome b6f (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well characterized, the interaction between PC and Cytf remains less clear. Using chemical cross-linking ...[more]

PMID: 40581738

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