Dataset Information

Proteomics analysis integrated with transcriptomics of clubroot resistant and susceptible Brassica napus near-isogenic lines in response to Plasmodiophora brassicae infection

ABSTRACT: Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat to Brassica crop production. Understanding the disease resistance mechanism is vital to assist in the continuous development of clubroot-resistant genetic material. In this study, we used clubroot-resistant (CR) and -susceptible (CS) near-isogenic lines (NILs) of B. napus, carrying resistance of turnip (B. rapa var. rapifera), and conducted transcriptomics and proteomics analysis to characterize the molecular basis of host resistance against clubroot. This study identified genes involved in reactive oxygen species (ROS) scavenging [peroxygenase 3 (BnaA04T0204000WE) and peroxidase 2 (BnaA09T0647200WE)], cell wall modifications [caffeoylshikimate esterase (BnaA04T0244300WE) and shikimate-O-hydroxycinnamoyltransferase (BnaC06T0472200WE)], and glucosinolate biosynthesis [2-oxoglutarate-dependent dioxygenase AOP1 (BnaA01T0266700WE)] that were highly abundant in CR lines. Additionally, disease resistance-related genes, including enhanced disease resistance 2-like (BnaA03T0055600WE) and hairpin-induced family protein YLS9 (BnaA08T0237900WE), were also elevated in the CR lines. In contrast, CS NILs exhibited decreased expression of defense-related genes, increased expression of cell wall-degrading enzymes, and enhanced carbohydrate metabolism, facilitating pathogen proliferation. Integrated analysis of data from the two ‘omics’ approaches showed that the genes related to plant invertase/pectin methylesterase inhibitor (BnaC04T0003100WE), Kelch motif (BnaC02T0374800WE), and laccase (BnaA02T0019200WE) potentially play an important role in host resistance. Antioxidant-related genes such as glucose-6-phosphate dehydrogenase (BnaC09T0029500WE), glutathione S-transferases (BnaA03T0281000WE, BnaA03T0280900WE), catalases (Bnascaffold286T0031400WE), and 4-hydroxyphenylpyruvate dioxygenase (BnaA09T0641500WE) were upregulated in the resistant NILs, potentially protecting cell wall components by ROS scavenging. The identified genes and pathways offer valuable targets for use in breeding aimed at enhancing clubroot resistance.

INSTRUMENT(S):

ORGANISM(S): Brassica Napus (rape)

TISSUE(S): Plant Cell, Root, Leaf

SUBMITTER: Richard Uhrig

LAB HEAD: R. Glen Uhrig

PROVIDER: PXD060262 | Pride | 2025-10-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20220811_Canola_Kawalpreet_Habib_01_L5_RC_14_1.raw	Raw
	20220811_Canola_Kawalpreet_Habib_02_L3_RC_7_3.raw	Raw
	20220811_Canola_Kawalpreet_Habib_03_L1_RC_7_1.raw	Raw
	20220811_Canola_Kawalpreet_Habib_04_L46_SI_21_2.raw	Raw
	20220811_Canola_Kawalpreet_Habib_05_L11_RC_21_3.raw	Raw

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Publications

Proteomics Integrated with Transcriptomics of Clubroot Resistant and Susceptible Brassica napus in Response to Plasmodiophora brassicae Infection.

Kaur Kawalpreet K Adhikary Dinesh D Kav Nat N V NNV Scandola Sabine S Uhrig R Glen RG Rahman Habibur H

International journal of molecular sciences 20250919 18

Clubroot disease, caused by Plasmodiophora brassicae, is a threat to Brassica crops; therefore, understanding of host-resistance is important for developing clubroot-resistant cultivars. Using multi-omics analysis of clubroot-resistant (CR) and -susceptible (CS) near-isogenic lines (NILs) of B. napus, carrying the resistance of turnip (B. rapa var. rapifera), we characterized the host resistance mechanisms. Through proteome analysis, we identified 6626 differentially ...[more]

PMID: 41009717

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Project description:Clubroot of Brassicaceae, an economically important soil borne disease, is caused by Plasmodiophora brassicae Woronin, an obligate, biotrophic protist. This disease poses a serious threat to canola and related crops in Canada and around the globe causing significant loss to seed yield. The pathogen is continuously evolving and new pathotypes are emerging, this necessitates the development of novel resistant canola cultivars to manage the disease effectively. Given that proteins play a crucial role in majority of biological processes and molecular functions, the identification of differentially abundant proteins (DAP) using proteomics information is an attractive approach to understand the plant-pathogen interactions as well as in the future development of gene specific markers for developing clubroot resistant (CR) cultivars. In this study, P. brassicae pathotype 3 (P3H) was used to challenge CR and clubroot susceptible (CS) canola lines. Root samples were collected at three distinct stages of pathogenesis, 7-, 14-, and 21-days post inoculation (DPI), protein samples were isolated, digested with trypsin and subjected to LC-MS/MS analysis. A total of 937 proteins demonstrated a significant (q < 0.05) change in abundance in at least in one of the time points when compared between control and inoculated CR-parent, CR-progeny, CS-parent, CS-progeny and 784 proteins were significantly (q < 0.05) changed in abundance in at least in one of the time points when compared between the inoculated- CR and CS root proteomes of parent and progeny across the three time points tested. Functional annotation of the differentially abundant proteins (DAPs) revealed several proteins related to calcium dependent signaling pathways in response to the pathogen. In addition, proteins related to reactive oxygen species (ROS) biochemistry, dehydrins, lignin, thaumatin, and phytohormones were identified. Among the DAPs, 74 putative proteins orthologous to CR proteins and quantitative trait loci (QTL) associated with eight CR loci in four chromosomes including chromosomes A3 and A8 were identified. In conclusion, these results have contributed to an improved understanding of the mechanisms that are involved in mediating response to P. brassicae in canola at the protein level.

Dataset Information

Proteomics analysis integrated with transcriptomics of clubroot resistant and susceptible Brassica napus near-isogenic lines in response to Plasmodiophora brassicae infection

Dataset's files

Publications

Proteomics Integrated with Transcriptomics of Clubroot Resistant and Susceptible <i>Brassica napus</i> in Response to <i>Plasmodiophora brassicae</i> Infection.

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Dataset Information

Proteomics analysis integrated with transcriptomics of clubroot resistant and susceptible Brassica napus near-isogenic lines in response to Plasmodiophora brassicae infection

Dataset's files

Publications

Proteomics Integrated with Transcriptomics of Clubroot Resistant and Susceptible &lt;i&gt;Brassica napus&lt;/i&gt; in Response to &lt;i&gt;Plasmodiophora brassicae&lt;/i&gt; Infection.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Proteomics Integrated with Transcriptomics of Clubroot Resistant and Susceptible <i>Brassica napus</i> in Response to <i>Plasmodiophora brassicae</i> Infection.