Proteomics analysis integrated with transcriptomics of clubroot resistant and susceptible Brassica napus near-isogenic lines in response to Plasmodiophora brassicae infection

ABSTRACT: Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat to Brassica crop production. Understanding the disease resistance mechanism is vital to assist in the continuous development of clubroot-resistant genetic material. In this study, we used clubroot-resistant (CR) and -susceptible (CS) near-isogenic lines (NILs) of B. napus, carrying resistance of turnip (B. rapa var. rapifera), and conducted transcriptomics and proteomics analysis to characterize the molecular basis of host resistance against clubroot. This study identified genes involved in reactive oxygen species (ROS) scavenging [peroxygenase 3 (BnaA04T0204000WE) and peroxidase 2 (BnaA09T0647200WE)], cell wall modifications [caffeoylshikimate esterase (BnaA04T0244300WE) and shikimate-O-hydroxycinnamoyltransferase (BnaC06T0472200WE)], and glucosinolate biosynthesis [2-oxoglutarate-dependent dioxygenase AOP1 (BnaA01T0266700WE)] that were highly abundant in CR lines. Additionally, disease resistance-related genes, including enhanced disease resistance 2-like (BnaA03T0055600WE) and hairpin-induced family protein YLS9 (BnaA08T0237900WE), were also elevated in the CR lines. In contrast, CS NILs exhibited decreased expression of defense-related genes, increased expression of cell wall-degrading enzymes, and enhanced carbohydrate metabolism, facilitating pathogen proliferation. Integrated analysis of data from the two ‘omics’ approaches showed that the genes related to plant invertase/pectin methylesterase inhibitor (BnaC04T0003100WE), Kelch motif (BnaC02T0374800WE), and laccase (BnaA02T0019200WE) potentially play an important role in host resistance. Antioxidant-related genes such as glucose-6-phosphate dehydrogenase (BnaC09T0029500WE), glutathione S-transferases (BnaA03T0281000WE, BnaA03T0280900WE), catalases (Bnascaffold286T0031400WE), and 4-hydroxyphenylpyruvate dioxygenase (BnaA09T0641500WE) were upregulated in the resistant NILs, potentially protecting cell wall components by ROS scavenging. The identified genes and pathways offer valuable targets for use in breeding aimed at enhancing clubroot resistance.

INSTRUMENT(S):

ORGANISM(S): Brassica Napus (rape)

TISSUE(S): Plant Cell, Root, Leaf

SUBMITTER: Richard Uhrig  

LAB HEAD: R. Glen Uhrig

PROVIDER: PXD060262 | Pride | 2025-10-06

REPOSITORIES: Pride