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PlxnA2 interactome in primary hippocampal murine neurons

ABSTRACT: Proper assembly of complex neural circuits requires the coordinated action of a limited set of axon guidance molecules, including plexins. To probe the extracellular vicinity of PlxnA2 in primary hippocampal neurons, we used in this work antibody directed proximity biotinylation and identified candidate co-receptors.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hippocampal Mossy Fiber, Brain

SUBMITTER: Juan Oses-Prieto

LAB HEAD: Roman J Giger

PROVIDER: PXD060323 | Pride | 2026-03-16

REPOSITORIES: Pride

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	files.xlsx	Xlsx
	searchresults-merged-PlxnA2KO-PlxnA2Het.xlsx	Xlsx

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Publications

Diverse and location-specific roles of PlexinA2, PlexinA4, and NCAM in developing hippocampal mossy fibers.

Zhao Xiao-Feng XF Kohen Rafi R Van Battum Eljo Y EY Niu Xiaoman X Zeng Ying Y Zhang Xiaolu X Johnson Craig N CN Wang Karen K Verhagen Marieke G MG Lim Brian C BC Oses-Prieto Juan A JA Rasband Joshua M JM Burlingame Alma L AL Cai Dawen D Pasterkamp R Jeroen RJ Rasband Matthew N MN Giger Roman J RJ

Translational psychiatry 20260212

Mossy fibers (MFs) originate from dentate granule cells and innervate area CA3 of the hippocampus. Upon entry of CA3, MFs partition into two prominent axon bundles, the suprapyramidal tract (SPT) and infrapyramidal tract (IPT) and form lamina specific synaptic contacts in the stratum lucidum (SL) and stratum oriens (SO), respectively. Here we mapped cell type specific gene expression and subcellular distribution of Sema6A and its receptors PlexinA2 (PlxnA2) and PlxnA4. Using a mouse genetic appr ...[more]

PMID: 41672969

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Analysis of NF186 proximity proteomes across neuronal development using Nfasc antibodies in an antibody-directed proximity biotinylation strategy combined with mass spectrometry

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The GAR Domain Determines Subcellular Localization of the Growth Controlling RNA Binding Protein Nucleolin

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Analysis of subcellular Importin b1 interactomes by BioID and BAR proximity labelling

Project description:Importin 1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in detection of importin complex cargos, and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable Biotinylation by Antibody Recognition (BAR) analysis of Importin 1 interactomes.

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IMP2 axonal localization, RNA interactome, and function in development of axon trajectories

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