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Hepa1-6 cell line overexpressing Flag-LTA4H LC-MS/MS

ABSTRACT: To explore potential proteins binding with LTA4H, co-immunoprecipitation (co-IP) and LC-MS/MS analysis were performed using Hepa1-6 cells overexpressing Flag-LTA4H. Anti-Flag and anti-IgG antibodies were used for immunoprecipitation to identify the interacting proteins.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Shuai Yang

LAB HEAD: Lei Chen

PROVIDER: PXD060326 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Flag_MS_identified_information.txt	Txt
	IgG_MS_identified_information.txt	Txt
	XA06995GI_Flag.raw	Raw
	XA06995GI_IgG.raw	Raw

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Publications

LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis.

Yang Shuai S Qiu Xinyao X Yang Yingcheng Y Wu Jing J Wang Shan S Zheng Bo B Wu Jianmin J Zhou Tao T Zhang Yangqianwen Y Bai Mixue M Liu Shuowu S Zhao Zihan Z Zhang Yani Y Wang Yixian Y Bao Jinxia J Wu Mengye M Xue Dongdong D Bao Meiyu M Hu Ji J Shen Siyun S Wang Hongyang H Chen Lei L

Cell reports. Medicine 20250307 3

Leukotriene A4 hydrolase (LTA4H), an inflammatory mediator, has garnered attention for its role in the development of chronic lung diseases and various cancers. Our study highlights the protective role of LTA4H in hepatocellular carcinoma (HCC) occurrence and progression. LTA4H is downregulated in clinical and mouse HCC tumors. LTA4H deficiency exacerbates hepatocyte damage by restraining JNK activation and promotes CD206<sup>+</sup> macrophage polarization through the upregulation of LTBP1 expr ...[more]

PMID: 40056904

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