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Metaproteomic analysis to assess the impact of storage media on human gut microbiome in fecal samples

ABSTRACT: We performed a mass spectrometry-based metaproteomic study to assess the impact of storage media on human gut microbiome in fecal samples. We evaluated FDA-authorized OMNIgene·GUT (OG), phosphate-buffered saline (PBS), and RNALater (RNAL) buffers.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Colon Cancer,Pancreatic Cancer

SUBMITTER: Van-An Duong

LAB HEAD: Ru Chen

PROVIDER: PXD060337 | Pride | 2025-10-13

REPOSITORIES: Pride

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			Action	DRS
	20210104_OG1_R1.raw	Raw
	20210104_OG1_R2.raw	Raw
	20210104_OG2_R1.raw	Raw
	20210104_OG2_R2.raw	Raw
	20210104_OG3_R1.raw	Raw

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Publications

Metaproteomic Analysis to Assess the Impact of Storage Media on Human Gut Microbiome in Fecal Samples.

Duong Van-An VA Pan Evan E Dabral Prerna P Utzschneider Kristina M KM Lampe Johanna W JW Chen Ru R A J Hullar Meredith M

Proteomics 20250828 19

The human gut microbiome is a diverse community of microorganisms residing in the gastrointestinal tract. The storage condition of fecal samples may impact the taxonomic and protein compositions of microbiomes in these samples. Here, we performed a mass spectrometry-based metaproteomic study to assess the impact of storage media on human gut microbiome in fecal samples. We evaluated FDA-authorized OMNIgene·GUT (OG), phosphate-buffered saline (PBS), and RNALater (RNAL) buffers and identified 38,1 ...[more]

PMID: 40873297

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