Dataset Information

Comparative analysis of platelet-derived extracellular vesicles protein extraction methodologies for mass spectrometry 

ABSTRACT: This study presents a comparative study of different methodologies for the extraction of proteins from platelet derived-extracellular vesicles (pEV) prior to subsequent mass spectrometry (MS) analysis. Protein isolation is performed through three different methodologies based on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), organic solvent precipitation (OSP) or magnetic beads (MB), followed by protein digestion and sample acquisition by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Platelet

SUBMITTER: Carmen Raez Meseguer

LAB HEAD: Joana Maria Ramis Morey

PROVIDER: PXD060357 | Pride | 2025-08-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	10_EVs_2_aillades_pEVs_2_1_B_1ul_cartutxo174500_col50_2h_1.raw	Raw
	13_EVs_3_aillades_pEVs_3_1_A_1ul_cartutxo174500_col50_2h_1.raw	Raw
	14_EVs_3_aillades_pEVs_3_1_B_1ul_cartutxo174500_col50_2h_1.raw	Raw
	17_Gel_EVs_1_primer_aillat_pEVs_1_1_A_1ul_cartutxo174500_col50_2h_1.raw	Raw
	18_Gel_EVs_1_primer_aillat_pEVs_1_1_B_1ul_cartutxo174500_col50_2h_1.raw	Raw

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Publications

Comparative Analysis of Platelet-Derived Extracellular Vesicle Protein Extraction Methodologies for Mass Spectrometry.

Ráez-Meseguer Carmen C Amengual-Tugores Andreu Miquel AM Forteza-Genestra Maria Antònia MA Orvay-Pintos Francisca F Gomila Rosa M RM Martorell-Crespí Gabriel G Calvo Javier J Gayà Antoni A Monjo Marta M Ramis Joana Maria JM

Journal of proteome research 20250721 8

The aim of this study is to present a comparative study of different methodologies for the extraction of proteins from platelet-derived extracellular vesicles (pEVs) prior to subsequent mass spectrometry (MS) analysis. pEVs were isolated by size exclusion chromatography (SEC) from human platelet lysates (PL) and characterized by identifying specific markers by Western blot, visualizing morphology by transmission electron microscopy (TEM) and analyzing concentration and size via nanoparticle trac ...[more]

PMID: 40691760

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