Comparative analysis of platelet-derived extracellular vesicles protein extraction methodologies for mass spectrometry
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ABSTRACT: This study presents a comparative study of different methodologies for the extraction of proteins from platelet derived-extracellular vesicles (pEV) prior to subsequent mass spectrometry (MS) analysis. Protein isolation is performed through three different methodologies based on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), organic solvent precipitation (OSP) or magnetic beads (MB), followed by protein digestion and sample acquisition by LC-MS/MS.
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Blood Platelet
SUBMITTER:
Carmen Raez Meseguer
LAB HEAD: Joana Maria Ramis Morey
PROVIDER: PXD060357 | Pride | 2025-08-25
REPOSITORIES: Pride
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