Dataset Information
CRISPR/dCAS9 genomic engineering for boosting the therapeutic potential of Extracellular Vesicles
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Stem Cell, Cell Culture
SUBMITTER: 
Iker Martinez Zalbidea
LAB HEAD: Karin Wuertz-Kozak
PROVIDER: PXD060381 | Pride | 2025-05-07
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| MartinezNTA24060.raw | Raw | |||
| MartinezNTB24060.raw | Raw | |||
| MartinezNTC24060.raw | Raw | |||
| MartinezTSA24060.raw | Raw | |||
| MartinezTSB24060.raw | Raw |
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Publications
CRISPR-dCas9 Activation of TSG-6 in MSCs Modulates the Cargo of MSC-Derived Extracellular Vesicles and Attenuates Inflammatory Responses in Human Intervertebral Disc Cells In Vitro.
Cellular and molecular bioengineering 20250205 1
<h4>Purpose</h4>The purpose of this study was to boost the therapeutic effect of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) by overexpressing the gene TSG-6 through CRISPR activation, and assess the biological activity of EVs from these modified MSCs <i>in vitro</i> on human intervertebral disc (IVD) cells.<h4>Methods</h4>An immortalized human MSC line was transduced with a CRISPR activation lentivirus system targeting TSG-6. MSC-EVs were harvested by ultracentrifugation an ...[more]
