Dataset Information

TRPV6 essentially contributes to Ca2+ influx in endometrial epithelial cells and female mouse fecundity

ABSTRACT: The Ca2+-selective transient receptor potential vanilloid 6 (TRPV6) channel plays a fundamental role in the mouse female and male reproductive system. In mouse endometrium the TRPV6 protein is highly expressed in epithelial cells (MEECs). Using Ca2+ imaging and patch clamp analysis we found that TRPV6 contributes to the cytosolic Ca2+ level, more specifically to the Ca2+ influx into the MEECs. As compared to wild-type cells, Trpv6-deficient MEECs reveal a significantly reduced frequency of spontaneous cytosolic Ca2+ oscillations, shown in both isolated cells and the complete uterus in situ. Noteworthy, in MEECs we here show for the first time distinct endogenous TRPV6-dependent whole-cell currents detected from primary cells. Our results disclose a previously unknown physiological role for TRPV6 in the regulation of endometrial Ca2+ homeostasis and its impact on female mouse fecundity, representing a basis for further investigation of reproductive impairments e.g. associated with defective Ca2+ regulation in women.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell

SUBMITTER: Claudia Fecher-Trost

LAB HEAD: Petra Weissgerber

PROVIDER: PXD060418 | Pride | 2025-10-13

REPOSITORIES: Pride

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TRPV6 channel function is involved in endometrial epithelial cell Ca2+ signaling and female mouse fecundity.

Sota Adela A Beck Andreas A Wartenberg Philipp P Gehl Anna-Lena AL Winter Manuel M Wissenbach Ulrich U Freichel Marc M Meyer Markus R MR Boehm Ulrich U Flockerzi Veit V Fecher-Trost Claudia C Weissgerber Petra P

Cellular and molecular life sciences : CMLS 20251007 1

The Ca2+-selective transient receptor potential vanilloid 6 (TRPV6) channel plays a fundamental role in the female and male murine reproductive system. We have previously shown that TRPV6 is essential for male fertility, and necessary for a proper placental Ca2+ transport, embryonic bone development and calcification, as well as for extracellular matrix formation in the placental labyrinth. Here, we show that lack of functional TRPV6 results in impaired fecundity in female ...[more]

PMID: 41055712

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Project description:Seminal fluid contains some of the fastest evolving proteins currently known. These seminal fluid proteins (Sfps) play crucial roles in reproduction, such as supporting sperm function, and – particularly in insects – modifying female physiology and behaviour. Identification of Sfps in small animals is challenging, and often relies on samples taken from the female reproductive tract after mating. A key pitfall of this method is that it might miss Sfps that are of low abundance due to dilution in the female-derived sample or rapid processing in females. Here we present a new and complimentary method, which provides added sensitivity to Sfp identification. We applied label-free quantitative proteomics to Drosophila melanogaster male reproductive tissue – where Sfps are unprocessed, and highly abundant – and quantified Sfps before and immediately after mating, to infer those transferred during copulation. We also analysed female reproductive tracts immediately before and after copulation to confirm the presence and abundance of known and candidate Sfps, where possible. Results were cross-referenced with transcriptomic and sequence databases to improve confidence in Sfp detection. Our data was consistent with 124 previously reported Sfps. We found 8 high-confidence novel candidate Sfps, which were both depleted in mated versus unmated males and identified within the reproductive tract of mated but not virgin females. We also identified 31 more candidates that are likely Sfps based on their abundance, known expression and predicted characteristics, and revealed that four proteins previously identified as Sfps are at best minor contributors to the ejaculate. The estimated copy numbers for our candidate Sfps were lower than for previously identified Sfps, supporting the idea that our technique provides a deeper analysis of the Sfp proteome than previous studies. Our results demonstrate a novel, high-sensitivity approach to the analysis of seminal fluid proteomes, whose application will further our understanding of reproductive biology.

			Action	DRS
	IPV6_MEEC_ALG118_1.mgf	Mgf
	IPV6_MEEC_ALG118_1.raw	Raw
	IPV6_MEEC_ALG118_2.mgf	Mgf
	IPV6_MEEC_ALG118_2.raw	Raw
	IPV6_MEEC_ALG118_3.mgf	Mgf

Dataset Information

TRPV6 essentially contributes to Ca2+ influx in endometrial epithelial cells and female mouse fecundity

Dataset's files

Publications

TRPV6 channel function is involved in endometrial epithelial cell Ca<sup>2+</sup> signaling and female mouse fecundity.

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Dataset Information

TRPV6 essentially contributes to Ca2+ influx in endometrial epithelial cells and female mouse fecundity

Dataset's files

Publications

TRPV6 channel function is involved in endometrial epithelial cell Ca&lt;sup&gt;2+&lt;/sup&gt; signaling and female mouse fecundity.

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OmicsDI is part of the ELIXIR infrastructure

Tweets

TRPV6 channel function is involved in endometrial epithelial cell Ca<sup>2+</sup> signaling and female mouse fecundity.