Dataset Information

Proteome and metabolome alterations in radish (Raphanus sativus L.) seedlings induced by inoculation with Agrobacterium tumefaciens

ABSTRACT: Here we provide a comprehensive explorative proteomics survey aiming at intensive charac-terization of metabolic shifts and molecular events accompanying plant responses to inoculation with A. tumefaciens culture. Based on the comprehensive proteomics dataset complemented with an untargeted metabolite profiling, we characterize the metabolic shifts, associated with agro-bacterial infection

INSTRUMENT(S):

ORGANISM(S): Raphanus Sativus

TISSUE(S): Seed

SUBMITTER: Daria Gorbach

LAB HEAD: Dr. Andrej Frolov

PROVIDER: PXD060463 | Pride | 2025-05-07

REPOSITORIES: Pride

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Proteome and Metabolome Alterations in Radish (Raphanus sativus L.) Seedlings Induced by Inoculation with Agrobacterium tumefaciens.

Frolova Nadezhda N Gorbach Daria D Ihling Christian C Bilova Tatiana T Orlova Anastasia A Lukasheva Elena E Fedoseeva Ksenia K Dodueva Irina I Lutova Lyudmila A LA Frolov Andrej A

Biomolecules 20250214 2

Infection of higher plants with agrobacteria (Agrobacterium tumefaciens) represents one of the most comprehensively characterized examples of plant-microbial interactions. Incorporation of the bacterial transfer DNA (T-DNA) in the plant genome results in highly efficient expression of the bacterial auxin, cytokinin and opine biosynthesis genes, as well as the host genes of hormone-mediated signaling. These transcriptional events trigger enhanced proliferation of plant cells and formation ...[more]

PMID: 40001593

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Project description:Agrobacterium tumefaciens, a bacterial species found in temperate soils world wide, is the causative agent of crown gall disease on many plants. A. tumefaciens-induced tumours are feared in orchards and vineyards because of their pathological interference with nutrient and water supply which results in crop decline. Small wounds at the crown of the plant, usually induced by wind-bending, are potential entry sites for the bacterium. The tumorous growth is initiated by the integration and expression of the T-DNA of the bacterial Ti plasmid within the plant nuclear DNA. The T-DNA encodes enzymes catalysing the synthesis of increased concentrations of auxin and cytokinin, and of opines which stimulate cell division and enlargement. The fast growing tumours have been shown to be a strong nutrient sink on their host plants. As a matter of fact, sugar and K+ content were found to be up to 10- and 5-fold, respectively, higher in this tissue and transpiration was about 15 times increased compared to normal tissue. Whereas the morphological structure as well as some physiological and biochemical parameters of the tumour have been analysed in detail, little is known about the underlying gene expression pattern. Proliferation and growth of the tumour induced by Agrobacterium tumefaciens is obviously due to the extraordinary high concentration of phytohormons, minerals and metabolites. Their influence on regulation of gene transcription will provide information on the mechanisms underlying fast tumour growth. In a project funded by the DFG we recently started to investigate the role of solute transporter for tumour development on the model plant Arabidopsis thaliana. By comparing the expression pattern of RNA preparations from Arabidopsis tumour and non-tumour tissue, we will be able to identify genes which facilitate crown gall development. For the expression analysis with an Affymetrix full genome chip we will induce tumours at the base of an injured Arabidopsis inflorescence stalk (var. Wassilewskija, WS-2). RNA will be extraxted from tumour and injured non-tumor inflorescence stalk tissue using the RNeasy Plant Mini Kit (Qiagen), followed by a DNase treatment to eliminate DNA contamination. Experimenter name = Rosalia Deeken; Experimenter phone = +49 931 8886121; Experimenter fax = +49 931 8886158; Experimenter institute = Julius-von-Sachs-Institute of Biosciences Molecular Plant Physiology and Electrophysiology; Experimenter address = Julius-von-Sachs-Platz 2; Experimenter address = Wuerzburg; Experimenter zip/postal_code = D-97082; Experimenter country = Germany Experiment Overall Design: 4 samples were used in this experiment

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Dataset Information

Proteome and metabolome alterations in radish (Raphanus sativus L.) seedlings induced by inoculation with Agrobacterium tumefaciens

Dataset's files

Publications

Proteome and Metabolome Alterations in Radish (<i>Raphanus sativus</i> L.) Seedlings Induced by Inoculation with <i>Agrobacterium tumefaciens</i>.

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Dataset Information

Proteome and metabolome alterations in radish (Raphanus sativus L.) seedlings induced by inoculation with Agrobacterium tumefaciens

Dataset's files

Publications

Proteome and Metabolome Alterations in Radish (&lt;i&gt;Raphanus sativus&lt;/i&gt; L.) Seedlings Induced by Inoculation with &lt;i&gt;Agrobacterium tumefaciens&lt;/i&gt;.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Proteome and Metabolome Alterations in Radish (<i>Raphanus sativus</i> L.) Seedlings Induced by Inoculation with <i>Agrobacterium tumefaciens</i>.