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Identification of Vha68-3 binding proteins by LC-MS/MS

ABSTRACT: To explore the regulatory network involving Vha68-3 binding proteins, immunocoprecipitation (IP) experiments were performed in S2 cells via LC-MS/MS analysis and Label-free quantification.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Yueshuai Guo

LAB HEAD: Jun Yu

PROVIDER: PXD060509 | Pride | 2025-05-26

REPOSITORIES: Pride

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	Ingel_IP_50_OE_20220328.raw	Raw
	Ingel_IP_ctrl_50_20220328.raw	Raw
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Publications

Multi-omics integration reveals Vha68-3 as a testicular aging-specific factor that coordinates spermatid elongation through mitochondrial metabolic homeostasis.

Yu Jun J Huang Qiuru Q Fu Yangbo Y He Lei L Shen Cong C Chen Xia X Li Zhiran Z Li Jiaxin J Wang Chenyu C Wang Xinda X Yang Binbin B Lin Ziwen Z Qiao Chen C Tan Xiaofang X Yang Xiaoqing X Chen Hao H Zheng Ying Y Zheng Bo B Sun Fei F

Cellular & molecular biology letters 20250509 1

<h4>Background</h4>Testicular aging has profound effects on spermatogenesis, sperm function, and the spermatogenic microenvironment, contributing to reduced male fertility. However, the precise molecular mechanisms by which mitochondria influence spermiogenesis during aging still remain largely unclear.<h4>Methods</h4>Vha68-3 KO flies were generated using the CRISPR/Cas9 technique. Testicular phenotypes and functions were mainly observed through immunofluorescence staining and transmission elect ...[more]

PMID: 40346547

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