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Characterization of human CD34+ HSC-derived neutrophils without myeloid-derived immunosuppressive cell activity

ABSTRACT: Neutrophils have limited utility as transfusion product due to their short lifespan. Culturing neutrophils from stem cell sources holds potential for fundamental and translational purposes. Here, we cultured CD34+ hematopoietic stem cell-derived neutrophils and compared them with peripheral blood neutrophils in terms of morphology, phenotype, and function. Our culture system resulted in 60% morphologically mature CD15+CD11b+CD16high neutrophils with most effector functions almost indistinguishable from blood neutrophils, confirmed by a high similarity in transcription and protein abundance patterns. While exhibiting strong microbial killing capacity and antibody-dependent cellular cytotoxicity against tumor cells, these cells were deficient in myeloid-derived suppressor cell activity. Upon dissecting the underlying mechanism, this deficiency in immunosuppressive activity correlated with their distinct granular composition in comparison to blood neutrophils. Taken together, our cultured neutrophils closely resemble blood neutrophils, offering a repository for fundamental research and a step towards an effective transfusion product without immunosuppressive activity.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neutrophil

SUBMITTER: Arie Hoogendijk

LAB HEAD: Arie Johan Hoogendijk

PROVIDER: PXD060579 | Pride | 2025-08-13

REPOSITORIES: Pride

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			Action	DRS
	MPB_11912_SLII_day0_S2-A1_3437.d.zip	Other
	MPB_12493_IMDM_day10_S2-G5_3486.d.zip	Other
	MPB_12493_IMDM_day14_bulk_S2-G6_3487.d.zip	Other
	MPB_12493_IMDM_day17_bulk_S2-G7_3488.d.zip	Other
	MPB_12493_SLII_day0_S2-B1_3444.d.zip	Other

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