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Identification of RNF187 binding proteins by LC-MS/MS

ABSTRACT: To explore the regulatory network involving RNF187 binding proteins, immunocoprecipitation (IP) experiments were performed in human spermatogonial stem cells (SSCs) via LC-MS/MS analysis and Label-free quantification.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yueshuai Guo

LAB HEAD: Bo Zheng

PROVIDER: PXD060599 | Pride | 2025-10-20

REPOSITORIES: Pride

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	Ingel_IP_GFP_20230811.raw	Raw
	Ingel_IP_RNF187_20230811.raw	Raw
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Publications

RNF187 Facilitates Proliferation and Migration of Human Spermatogonial Stem Cells Through WDR77 Polyubiquitination.

Hu Haoyue H Xi Xiaoxue X Jiang Bing B Wang Kehan K Wu Tiantian T Chen Xia X Guo Yueshuai Y Zhou Tao T Huang Xiaoyan X Yu Jun J Gao Tingting T Wu Yibo Y Zheng Bo B

Cell proliferation 20250408 10

The E3 ubiquitin ligase RNF187, also known as RING domain AP1 coactivator-1, is a member of the RING finger family. RNF187 is indispensable for the proliferation and migration of GC-1 cells derived from mouse spermatogonia and GC-2 cells derived from spermatocytes. However, it remains unclear whether RNF187 plays a crucial role in the self-renewal and migration of human spermatogonial stem cells (SSCs). In this study, we observed a positive correlation between RNF187 expression and the prolifera ...[more]

PMID: 40197797

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