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Persistent mirusvirus infection in the marine protist Aurantiochytrium

ABSTRACT: The protist Aurantiochytrium limacinum possesses two mirusvirus-like genomic elements, one a circular episome (AurliV-1) and the other (AurliV-2) a chromosomal integrant. Here we show that genes in both genomes are expressed and virions containing mainly AurliV-1 DNA are produced under starvation conditions and when cells are cultured in standard growth medium. Of 67 AurliV-1-encoded proteins detected using proteomics, 48 are enriched under starvation conditions, including the structurally important major capsid and triplex proteins. Our results establish Aurantiochytrium as a model system for elucidating mirusvirus-host interactions and demonstrate persistent viral infection in a microbial eukaryote.

INSTRUMENT(S):

ORGANISM(S): Aurantiochytrium Limacinum Atcc Mya-1381

TISSUE(S): Cell Line Cell

SUBMITTER: Nikolaj Brask

LAB HEAD: John Archibald

PROVIDER: PXD060676 | Pride | 2025-09-23

REPOSITORIES: Pride

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			Action	DRS
	240516_1196_md_nuclei_dda1_20240522132329.mzML	Mzml
	241203_SampleMatrix.txt	Txt
	Chung_Figures_and_Analysis.xlsx	Xlsx
	DDA_fragpipe_results.tsv	Tabular
	DIA.pg_matrix.tsv	Tabular

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