Dataset Information

Nucleus Accumbens proteome disbalance in an adolescent mice model of schizophrenia and nicotine misuse comorbidity

ABSTRACT: Considering the role of the nucleus accumbens (NAcc) as a common neurobiological substrate for the interplay between these psychiatric disorders, label-free proteomics was employed to identify NAcc deregulated proteins in male and female mice modeled to schizophrenia with a history of adolescent nicotine exposure. Phencyclidine was used to model schizophrenia and minipump infusions was used to model nicotine misuse. Enrichment Reactome pathway and protein–protein interaction analyses revealed the cytoskeleton and associated synaptic plasticity mechanisms, energy metabolism, and nervous system development, as affected in both females and males modeled to the comorbid condition.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Tissue Development

DISEASE(S): Schizophrenia

SUBMITTER: Andres Rodriguez Vega

LAB HEAD: Andres Rodriguez Vega

PROVIDER: PXD060733 | Pride | 2025-05-13

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20220117_2_34_003.raw.zip	Raw
	20220117_2_38_001.raw.zip	Raw
	20220117_2_38_002.raw.zip	Raw
	Results_PRIDE.DerivedFromSearchdd86b5ae-6b65-43cf-8def-1a59dae88b25.mgf	Mgf
	Results_PRIDE.mzid	Mzid

Items per page:

1 - 5 of 5

Publications

Nucleus Accumbens Proteome Disbalance in an Adolescent Mouse Model of Schizophrenia and Nicotine Misuse Comorbidity.

Souza Thainá Pereira TP Rodríguez-Vega Andrés A Dutra-Tavares Ana Carolina AC Semeão Keila A KA Filgueiras Claudio Carneiro CC Ribeiro-Carvalho Anderson A Manhães Alex Christian AC Abreu-Villaça Yael Y

Biomedicines 20250408 4

<b>Background/Objectives</b>: Schizophrenia and nicotine misuse are a comorbid condition that frequently develops during adolescence. Considering the role of the nucleus accumbens (NAcc) as a common neurobiological substrate for these psychiatric disorders, label-free proteomics was employed to identify NAcc deregulated proteins in male and female mouse models of schizophrenia with a history of adolescent nicotine exposure. <b>Methods</b>: Phencyclidine was used to model schizophrenia, and minip ...[more]

PMID: 40299488

Similar Datasets

Project description:Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. The PCM is a systemic disease caused by the thermo dimorphic fungus Paracoccidioides spp, which can exist as mycelia and yeast forms. The mycelia form can be found in environment at temperatures between 18 °C and 25 °C and produces spores or conidia. The PCM infection route occurs by the inhalation of conidia. Once in the lungs, these particles are converted into yeasts pathogenic form. Until now, metabolic aspects and virulence factors related to Paracoccidioides lutzii infective propagule are little understood. In this concern, a global proteomic study of P. lutzii conidia was performed. For conidia production, the mycelia of the isolate Pb01 were cultured in potato agar medium during 90 days at 18 °C. Obtained conidia were collected and purified. The proteins were extracted and subjected to tryptic digestion followed by identification by NanoUPLC-MSE approach. We identified a total of 242 proteins in P. lutzii conidia which were subjected to in silico functional analysis Proteins putatively acting as adhesins, such as GAPDH, enolase, and the glycoprotein β-1,3-glucanosyltransferase (gel2) were identified during our analysis. We also identified proteins related to signal transduction pathways, such as Ras and RhoA GTPases, previously demonstrated to be required for morphologic changes in pathogenic fungi. Proteins related to evasion, defense and virulence were also identified. Proteins acting during temperature shifts or oxidative stress provided by the host environment such as HSP90, catalase, mitochondrial peroxiredoxin Prx1 were identified. It were also identified proteins related to energy production and protein biosynthesis, which can provide important aspects to survival of this resting cells. These results highlight that P. lutzii conidia contain proteins that can contribute to its maintenance in the environment and also molecules related to host-pathogen interactions.