Dataset Information

The mass spectrometry analysis detected differences in protein lactylation in B16 cells after lactic acid treatment.

ABSTRACT: In this experiment, we treated the B16 cell line with lactic acid and performed peptide immunoprecipitation using anti-lactylation antibodies. We then detected the overall lactylation modifications of intracellular proteins after lactic acid treatment using mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Melanocyte, Cell Culture

DISEASE(S): Melanoma And Neural System Tumor Syndrome

SUBMITTER: pu wang

LAB HEAD: pu wang

PROVIDER: PXD060840 | Pride | 2025-07-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	YUC-1.tdf_bin	Other
	YULA-1.tdf_bin	Other
	identification_results_produced_by_the_analysis_tool.xlsx	Xlsx

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Publications

Lactic acid inhibits melanin synthesis by regulating histone H3 lactylation and suppressing tyrp1 transcription in B16 melanoma cells.

Du Bin B Xiao Meng M Gao Liang L

Scientific reports 20250716 1

Lactic acid, a widely distributed metabolic intermediate, exhibits superior permeability and mild acidity, making it a common reagent in skincare products. Lactic acid could significantly reduce melanin synthesis both in vitro and in vivo. However, the underlying molecular mechanisms remain less clear. To reveal the molecular mechanism by which lactic acid inhibits melanin synthesis through epigenetic pathways, proteins with significantly increased levels of lactylation modifications after lacti ...[more]

PMID: 40670430

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