HDX-MS analysis of a light-oxygen-voltage sensor-containing histidine kinase

ABSTRACT: The general stress response (GSR) protects bacteria from a wide range of stressors. In Alphaproteobacteria, GSR activation is coordinated by HWE/HisKA2 family histidine kinases (HKs), which can exhibit non-canonical structure and function. For example, while most light-oxygen-voltage sensor-containing HKs are light activated dimers, the Rubellimicrobium thermophilum RT-HK has inverted “dark on, light off” signaling logic with a tunable monomer/dimer equilibrium. Here, we further investigate these atypical behaviors of RT-HK and characterize its downstream signaling network. Using hydrogen-deuterium exchange mass spectrometry, we find that RT-HK uses a signal transduction mechanism similar to light-activated systems, despite its inverted logic.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Kevin Gardner  

LAB HEAD: Kevin H. Gardner

PROVIDER: PXD060849 | Pride | 2025-06-16

REPOSITORIES: Pride