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Order 28341 - Carla Miro Vinyals 2022-05-09

ABSTRACT: HeLa treated with IAA or NEM (Figure S3). Stable cell line expressing GALT1-HA-sCGrx1p sensor protein were treated with 20 mM NEM (A) or IAA (B) for 30 min at 37°C. The processed samples are anti-HA magnetic beads with the sensor protein captured from whole cell lysate.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tobias Kockmann

LAB HEAD: Pablo Rivera

PROVIDER: PXD060873 | Pride | 2025-02-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20220520_C28341_002_S385773_HeLa_Galt1HAsCGrx1p_C4_NEM_A1_Recombinant_protein.raw	Raw
	20220520_C28341_004_S385774_HeLa_Galt1HAsCGrx1p_C4_IAA_B_Recombinant_protein.raw	Raw
	checksum.txt	Txt
	o28341_minLib.sky.zip	Other

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Publications

Characterization of the glutathione redox state in the Golgi apparatus.

Miró-Vinyals Carla C Emmert Sarah S Grammbitter Gina G Jud Alex A Kockmann Tobias T Rivera-Fuentes Pablo P

Redox biology 20250219

Redox homeostasis is crucial for cell function, and, in eukaryotic cells, studying it in a compartmentalized way is essential due to the redox variations between different organelles. The redox state of organelles is largely determined by the redox potential of glutathione, E<sub>GSH</sub>, and the concentration of its reduced and oxidized species, [GS]. The Golgi apparatus is an essential component of the secretory pathway, yet little is known about the concentration or redox state of GSH in th ...[more]

PMID: 39986117

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Order 30968 - Carla Miro Vinyals 2023-02-23, Order 35373 - Carla Miro Vinyals 2024-05-29

Project description:HeLa or HEK293 cells transiently transfected with Galt1-sCGrx1p-HA redox sensor protein. Cells were treated with 0.1 mM IAA for 20 min on ice. The samples are anti-HA magnetic beads with the sensor protein captured from whole cell lysate.

2025-02-19 | PXD060874 | Pride

Transcription profiling of Saccharomyces cerevisiae pGAL1-MKS1, pGAL1-NNK1, and pGAL1-FMP48 overexpression

Project description:Analysis of the transcriptional effects of pGAL1-MKS1, pGAL1-NNK1, and pGAL1-FMP48 overexpression. BY4700 gal1::NAT ura3delta0 S. cerevisiae cells carrying a [pGAL1-MKS1-HA, URA3, CEN], [pGAL1-NNK1-HA, URA3, CEN], or [pGAL1-FMP48-HA, URA3, CEN] plasmid, or empty vector BG1805, were grown to early log phase, induced with 0.2% galactose, and harvested after 1.5 h and 6 h. RNA from a strain overexpressing MKS1, NNK1, or FMP48 was competitively hybridized with RNA from an identically treated strain carrying empty vector.<br><br>FMP48 = YGR052W. MKS1 = YNL076W. NNK1 = YKL171W.

2010-05-25 | E-MEXP-2171 | biostudies-arrayexpress

Obtusaquinone is a cysteine modifying compound that targets Keap1 for degradation

Project description:Bovine catalase: This is an experiment to use differential cysteine alkylation to map the nature of binding of a cysteine-targeting small molecule OBT (for details of the method, please see the Method Section of the manuscript). The alkylating reagents uses besides the studied drug were iodoacetamide (IAA) and N-ethylmaleimide (NEM). As described in the manuscript the experiment includes three routes of serial alkylation experiments termed OBT, IAA, and NEM. Samples were prepared in triplicate and TMT10-plex reagents were used for labeling as follows: OBT replicate 1 (OBT1), 129c; OBT2, 130n; OBT3, 130c; IAA1, 126; IAA2, 127n; IAA3, 127c; NEM1, 128n; NEM2, 128c; NEM3, 129n. An SPS-MS3 method was used for data acquisition on an Orbitrap Fusion Lumos mass spectrometer. The RAW file for these data is catalase_diffAlk.RAW. A differential alkylation experiment was also performed as described above for bovine catalase but using recombinant human KEAP1 as substrate. One set of experiments (OBT, IAA, NEM) was performed. TMT10-plex labeling was performed as follows: OBT, 129n; IAA, 128c; NEM, 128n. An SPS-MS3 method was used for data acquisition on an Orbitrap Fusion mass spectrometer. The RAW file for these data is RAW_file: keap1_diffAlk.RAW.

2019-11-30 | MSV000084638 | MassIVE

Expression data from oxidant treated yeast

Project description:Yeast transcription factor Yap1 mediates adaptive response against H2O2 and the cystein thiol reactive Michael acceptor, N-ethylmaleimid (NEM) and acrolein. The response against H2O2 was found to be distinct from that against NEM and acrolein. We used microarray experiment to identify two subsets of Yap1-dependent genes that correspond to these two adaptive responses. Wild-type yeast BY4741 cells were grown in early exponential phase and treated with H2O2, NEM, or acrolein. RNAs were then extracted and hybridized on Affymetrix microarrays.

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Posttranslation modification of NF-kB inducing kinase

Project description:2 This project is to identify the potential neddylation sites in human NF-kB inducing kinase (A.k.a. Map3K14). NIK-HA or NIK-HA in combination with FLAG-NEDD8 were co-expressed in HEK293T cells. NIK-HA were pulled down via denaturing immunoprecipitation protocol, subject to SDS-PAGE, in-gel trypsin digestion, and Nano LC-MS/MS Analysis. The potential peptides containing K-GG sites were identified.

2023-03-10 | PXD038653 | Pride

Transcription profiling of hyperthermophilic crenarchaea Sulfolobus solfataricus and Sulfolobus acidocaldarius to UV irradiation

Project description:Analysis of transcriptional response to UV irradiation in two related crenarchaea, Sulfolobus solfataricus and Sulfolobus acidocaldarius.

2007-11-21 | E-MEXP-1252 | biostudies-arrayexpress

Transcription profiling of synchronized Sulfolobus acidocaldarius populations as they progress through the cell cycle

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2007-12-08 | E-MEXP-936 | biostudies-arrayexpress

modENCODE RNA-Seq of Drosophila cell lines Kc167, CME_W1_Cl.8+, S2-DRSC and ML-DmBG3-c2

Project description:Deep Sequencing of mRNA from the Drosophila melanogaster cell lines Kc167, CME_W1_Cl.8+, S2-DRSC and ML-DmBG3-c2. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Analysis of poly(A)+ RNA from 2 biological replicates of Kc167, CME_W1_Cl.8+, S2-DRSC and ML-DmBG3-c2 cell lines.

2009-04-08 | E-GEOD-15596 | biostudies-arrayexpress

Transcription profiling time series of thymocytes from HNT-TCR transgenic mice injected with HA peptide to induce thymic negative selection

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Transcriptional profiling of a slyA mutant and a slyA-overexpression strain of Escherichia coli MG1655 in order to elucidate the SlyA regulon

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