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Mapping the intracellular HMGB1 interactome and alterations induced by Toll-like receptor 4 activation

ABSTRACT: THP1 cells were transduced with MycBioID2-HMGB1 and thereafter cultured in SILAC media for 1 week. Cells were treated with doxycycline (2 ug/ml, 48h) to induce gene expression. Thereafter, doxycycline was removed and heavy labelled cells were stimulated with LPS (100 ng/ml) for 1 hour before addition of biotin (24 hours). Light cells were used as control. Biotinylated proteins were extracted and identified with LC-MS/MS

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte

SUBMITTER: Rebecka Heinbäck

LAB HEAD: Helena Erlandsson

PROVIDER: PXD060878 | Pride | 2025-11-11

REPOSITORIES: Pride

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	N1ForPride.xlsx	Xlsx
	N2ForPride.xlsx	Xlsx
	N3ForPride.xlsx	Xlsx
	RawData_n1.mzML	Mzml
	RawData_n1.raw	Raw

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Mapping the intracellular HMGB1 interactome and alterations induced by Toll-like receptor 4 activation.

Heinbäck Rebecka R van der Zijde Marit M Tsang Choi Har CH Tršelič Tilen T Espinosa Alexander A Aulin Cecilia C Erlandsson Harris Helena H

The Journal of biological chemistry 20251027

The evolutionary conserved, mammalian protein HMGB1 is involved in chromatin-related mechanisms, autophagy and in sensing of immunogenic DNA. HMGB1 translocates from the nucleus to the cytosol in stressed myeloid cells, implicating functional changes. However, the role of intracellular HMGB1 in homeostatic conditions and alterations caused by stress remains largely unexplored. To better understand the intracellular roles of HMGB1, we defined the HMGB1 interactome in resting and LPS-stressed mono ...[more]

PMID: 41161382

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