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Variation in Plasma Proteome Associated with Delayed Blood Processing

ABSTRACT: Variations in pre-analytical processing of plasma samples can potentially result in different proteomic profiles identifed by downstream analyses. Here, we investigated the effects of delayed blood processing for up to eight hours on the observed plasma protein groups by tandem mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Xi Peng

LAB HEAD: Kunhong Xiao

PROVIDER: PXD060901 | Pride | 2026-01-05

REPOSITORIES: Pride

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			Action	DRS
	ProteinGroup_NoFilters.txt	Txt
	XP_02102023_Ind3_0hr_10nLPlasma_Rep1_Column4_88min_DIA_S1-A1_1_1932.d.zip	Other
	XP_02102023_Ind3_0hr_10nLPlasma_Rep2_Column4_88min_DIA_S1-A2_1_1934.d.zip	Other
	XP_02102023_Ind3_0hr_10nLPlasma_Rep3_Column4_88min_DIA_S1-A3_1_1936.d.zip	Other
	XP_02102023_Ind3_4hr_10nLPlasma_Rep1_Column4_88min_DIA_S1-B1_1_1938.d.zip	Other

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Publications

Peptide-Level Biomarker as a New Direction for Blood-Based Testing: Evaluation of Plasma Proteome Variability Induced by Prolonged on-Ice Storage.

Peng Xi X Zhang Qiangmin Q Omstead Ashten A Mansoor Rubab R Laframboise William W Zaidi Ali H AH Wagner Patrick L PL Bartlett David D Xiao Kunhong K

Journal of proteome research 20251216 1

Plasma proteomics has been explored extensively for biomarker discovery for both diagnostic and therapeutic purposes. The gold standard for plasma proteomics sample preparation requires immediate plasma isolation following blood collection, which poses logistical challenges in many clinical settings. To evaluate the feasibility of delayed plasma processing, we investigated the impact of prolonged on-ice storage (i.e., 0/4/8 h postcollection) on plasma proteomic profiles using a liquid chromatogr ...[more]

PMID: 41401394

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