Dataset Information

Caspase-8 expression and its Src dependent phosphorylation on Tyr380 triggers NRF2 signaling activation in glioblastoma

ABSTRACT: Here we have employed quantitative mass spectrometry based phosphoproteomics and proteomics to characterise the impact of knocking-down the caspase 8 gene in glioblastoma cells. This strategy enabled the identification of the pathways and processes modulated by caspase 8.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Francesca Sacco

LAB HEAD: Daniela Barila

PROVIDER: PXD060920 | Pride | 2025-12-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2024-05-09_MS_EXT_FS_shCaspase8_Ctrl_01.raw	Raw
	2024-05-09_MS_EXT_FS_shCaspase8_Ctrl_02.raw	Raw
	2024-05-09_MS_EXT_FS_shCaspase8_Ctrl_03.raw	Raw
	2024-05-09_MS_EXT_FS_shCaspase8_Ctrl_04.raw	Raw
	2024-05-09_MS_EXT_FS_shCaspase8_IR_01.raw	Raw

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Publications

Caspase-8 expression and its Src dependent phosphorylation on Tyrosine 380 triggers NRF2 signaling activation in glioblastoma.

Cirotti Claudia C Di Girolamo Claudia C Taddei Irene I Contadini Claudia C Massacci Giorgia G Sacco Francesca F Del Bufalo Donatella D Salvatori Illari I Valle Cristiana C Barilà Daniela D

Cell death and differentiation 20251006 12

Caspase-8 expression is upregulated in many tumors where, despite its canonical apoptotic pathway, it sustains cancer progression promoting cell migration, NF-kB activation and inflammation. Here, we provide the first evidence for a novel role of Caspase-8 in promoting the metabolic rewiring of cancer cells. By performing transcriptomic, proteomic and phosphoproteomic analyses on glioblastoma cellular models, we identify Caspase-8 as an unexpected modulator of NRF2. Here we show that Caspase-8 e ...[more]

PMID: 41053176

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