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Tandem affinity purification for sigma3 interacting host proteins

ABSTRACT: In order study the mechanism of how sigma3 regulates NF-kB activation, we performed tandem affinity purification to identify its host interacting proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture

SUBMITTER: Qin Fu

LAB HEAD: Qin Fu

PROVIDER: PXD061062 | Pride | 2026-02-23

REPOSITORIES: Pride

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			Action	DRS
	Yingying10399539_pNTAP_neg.mgf	Mgf
	Yingying10399539_pNTAP_neg.msf	Msf
	Yingying10399539_pNTAP_neg.raw	Raw
	Yongying10399539_pNTAP_Sigma3.mgf	Mgf
	Yongying10399539_pNTAP_Sigma3.msf	Msf

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1 - 5 of 7

Publications

A dsRNA Viral Transcriptional Regulator Evades Innate Immunity by Hijacking Host CoTranscription Factor DHX9.

Pang Xueyang X Liu Shiyu S Zhu Yixiao Y Hinchman Meleana M MM Qiao Nan N Li Xuejun X Dai Weiqian W Tong Yingying Y Xing Man M Ren Jiling J Parker John S L JSL Guo Yingying Y Zhou Dongming D

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20251222

Viral transcriptional regulators (vTRs) have emerged as potent factors that shape the host anti-viral gene expression programs. Delineating the molecular mechanisms by which vTRs inhibit or promote transcription will provide fundamental insights for developing anti-viral strategies. Using Mammalian orthoreovirus (REOV) as a model system, a new viral mechanism by which viruses antagonize the host innate immune response is identified. We found that the REOV outer capsid protein σ3 functions as a v ...[more]

PMID: 41431149

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A novel YDDX linear motif mediates docking of the IKK kinase to substrates: structural and functional insights

Project description:The inhibitor of kB kinase (IKK) is the master regulator of the nuclear factor kB (NF-kB) pathway, involved in inflammatory, immune and apoptotic responses. In the ‘canonical’ NF-kB pathway, IKK phosphorylates inhibitor of kB (IkB) proteins and this triggers ubiquitin-mediated degradation of IkB, leading to release and nuclear translocation of NF-B transcription factors. The data presented show that the IKK and IKK subunits recognize a YDDX docking site located within the disordered C-terminal region of IkBa. Our results also suggest that IKK contributes to the docking interaction with higher affinity as compared to IKK.

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Identification of cellular binders of human MX2 through a 3-labels SILAC based experiment using 293T cells protein over-expression and affinity purification

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The NF-kB genomic landscape in lymphoblastoid B-cells

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Chromatin tandem affinity purification sequencing.

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