Dataset Information

Enhancing tandem MS sensitivity and peptide identification via ion pre-accumulation in an Orbitrap mass spectrometer

ABSTRACT: High-throughput mass spectrometry-based proteomics is gaining increasing interest, but mass spectrometers need to adapt to enable faster analysis times. For Orbitrap mass spectrometers, faster scan rates are constrained by limitations on ion accumulation time, duty cycle, and transient length. Here, we introduce a new scanning strategy called "pre-accumulation" that enables storing ions in the bent flatapole in parallel with the C-trap/IRM operation. This significantly improves ion beam utilization and allows scanning speeds over 70 Hz on hybrid Orbitrap instruments.The combination of pre-accumulation and increased scan speeds enhances peptide and protein group identifications for short LC gradients, and improves sensitivity for high-throughput applications. These benefits are further amplified when coupled with full mass range phase-constrained spectrum deconvolution (ΦSDM), especially for fast, lower-resolving Orbitrap measurements. The pre-accumulation approach offers distinct advantages, particularly for conditions with reduced signal input, and does not require any hardware changes, making it highly attractive for Orbitrap mass spectrometers used with fast MS/MS acquisition methods.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Ulises H Guzman

LAB HEAD: Jesper V.

PROVIDER: PXD061135 | Pride | 2026-02-16

REPOSITORIES: Pride

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			Action	DRS
	20240130_EXPL5_EVO7_FH_SA_HeLa_0ng_sRNG_Ptoff_fdsm2_01.raw	Raw
	20240130_EXPL5_EVO7_FH_SA_HeLa_25ng_sRNG_Ptoff_fdsm0_01.raw	Raw
	20240130_EXPL5_EVO7_FH_SA_HeLa_25ng_sRNG_Ptoff_fdsm0_02.raw	Raw
	20240130_EXPL5_EVO7_FH_SA_HeLa_25ng_sRNG_Ptoff_fdsm0_03.raw	Raw
	20240130_EXPL5_EVO7_FH_SA_HeLa_25ng_sRNG_Ptoff_fdsm2_01.raw	Raw

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Publications

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer.

Guzman Ulises H UH Rykær Martin M Hendriks Ivo A IA Stewart Hamish H Denisov Eduard E Hagedorn Bernd B Petzoldt Johannes J Kreutzmann Arne A Mueller Yannick Y Arrey Tabiwang N TN Colonius Immo I Østergaard Ole O Koenig Claire C Kraegenbring Julia J Fort Kyle L KL Couzijn Erik E Hauschild Jan-Peter JP Hermanson Daniel D Zabrouskov Vlad V Hock Christian C Damoc Eugen E Olsen Jesper V JV

Molecular & cellular proteomics : MCP 20260109 2

High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems biology. However, current mass spectrometry technologies face limitations in speed, sensitivity, and scalability for analyzing large sample cohorts. The Thermo Scientific Orbitrap Astral Zoom mass spectrometer (MS) was developed to address these limitations by improving acquisition speed, ion utilization, and spectral processing, which are all essent ...[more]

PMID: 41520856

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