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Neo N-termiome of SPG7, AFG3L2, SPG7 AFG3L2 and WT HeLa cells

ABSTRACT: To understand how OMA1 affects mitochondrial proteostasis and stress responses, we performed a proteomic survey of OMA1-deficient cells for proteolytic substrates. We demonstrate that OMA1 cleaves the mitochondrial chaperone DNAJC15 facilitating its degradation by the mitochondrial m-AAA protease. The loss of DNAJC15 alters protein import by TIM23 protein translocases in the IM and limits the accumulation of OXPHOS-related mitochondrial matrix and IM proteins. Non-imported mitochondrial preproteins accumulate at the endoplasmic reticulum (ER) and trigger an ATF6-related unfolded protein response. These results demonstrate that OMA1 allows to adapt mitochondrial protein biogenesis to stress and reveal an intricate network of cellular stress responses to proteostasis disturbances. In this dataset, we determined the Neo N termiome of SPG7, AFG3L2 and SPG7 AFG3L2 knock out HeLa cells. The project is related to other identfiers PXD061131, PXD061134.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Hendrik Nolte

LAB HEAD: Thomas Langer

PROVIDER: PXD061137 | Pride | 2025-12-19

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	0066_sample_description.xlsx	Xlsx
	20191229_HN_0066_YvLa_rep_01_NeoN_sample_01.raw	Raw
	20191229_HN_0066_YvLa_rep_01_NeoN_sample_02.raw	Raw
	20191229_HN_0066_YvLa_rep_01_NeoN_sample_03.raw	Raw
	20191229_HN_0066_YvLa_rep_01_NeoN_sample_04.raw	Raw

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