Dataset Information

Exoproteome of clinical isolates of Pseudomonas aeruginosa with limited cheating ability

ABSTRACT: Pseudomonas aeruginosa is an opportunistic bacterial pathogen that possesses a plethora of virulence factors, including the public goods siderophores and exoproteases. Mutants unable to produce either exoprotease or siderophores are selected in competition with producers; hence, these two virulence factors are prone to exploitation by social cheaters. However, so far most of the studies of exoprotease exploitation by social cheaters had been done in the reference strains PAO1 and PA14 and the information available of these behaviors in clinical or environmental isolates is limited to a few studies. In this work, we studied the exoproteases secreted by two clinical isolates from cystic fibrosis patients belonging to the epidemic clone ST274-CC274. We found that the exoprotease exploitation in these strains is mild and not a result of the selection of lasR mutants during continuous growth in casein as sole carbon source, in contrast with the paradigm in the reference strains.

INSTRUMENT(S):

ORGANISM(S): Pseudomonas Aeruginosa Pao1h2o Bacteria

SUBMITTER: Hector Quezada

LAB HEAD: Rodolfo Garcia Contreras

PROVIDER: PXD061353 | Pride | 2025-11-10

REPOSITORIES: Pride

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Publications

Challenging the paradigm: non-canonical exoprotease cheating in clinical Pseudomonas aeruginosa isolates.

Guadarrama-Orozco Katya Dafne KD Esquivel-Hernández Diego Armando DA Islas-Tolentino Miguel Ángel MÁ Husain Fohad Mabood FM Quezada Héctor H García-Reyes Selene S Franco Bernardo B Marroquin-Mendiola Diana Laura DL Lucero-Gil María Guadalupe MG Olvera-Falfan Lorena Paola LP Estrada-Velasco Ángel Yahir ÁY Fabián Del Olmo Misael Josafat MJ Cocotl-Yañez Miguel M Tomas María M Peña-Ocaña Betsy Anaid BA Maeda Toshinari T Khan Altaf A Arshad Mohammed M Cantón Rafael R Oliver Antonio A Kidd Timothy J TJ Valdez Alejandra A Cadet Frederic F Toya Shotaro S Fontaine Nicolas N Ceapă Corina-Diana CD Kirigo Joy J Wood Thomas K TK García-Contreras Rodolfo R

FEMS microbiology ecology 20251001 11

Pseudomonas aeruginosa is a model organism for studying social behaviors in bacteria, such as the exploitation of exoprotease by social cheaters. The current paradigm holds that continuous culture of exoprotease-producing individuals with protein as the sole carbon source selects for exoprotease non-producers mutants with an impaired quorum-sensing regulator, LasR, which controls exoprotease expression. However, recent studies reveal that some isolates lacking functional LasR still produce exopr ...[more]

PMID: 41124041

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