Proteomics

Dataset Information

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Dynamic SILAC NIH/3T3 treated with DMSO/CHX


ABSTRACT: NIH/3T3 cells were cultured at least 2 weeks in light medium: DMEM for SILAC (Thermo scientific) supplemented with PS, dialyzed FBS (Thermo scientific), (light) L-Lysine-2HCl (0.666 mM), (light) L-Arginine-HCl (0.399 mM)44, and L-Proline (200 mg/L). Before switching from light to heavy medium, cells were treated with 0.05 µg/mL CHX or equivalent dilution of DMSO in 6-well plates. At timepoint 0 h, we replaced light medium with pre-warmed heavy medium: DMEM for SILAC (Thermo scientific) supplemented with PS, dialyzed FBS (Thermo scientific), (heavy) 13C6 15N2 L-Lysine-2HCl (0.666 mM), (heavy) 13C6 15N4 L-Arginine-HCl (0.399 mM), and L-Proline (200 mg/L) for NIH/3T3.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Benjamin Martin  

LAB HEAD: David M. Suter

PROVIDER: PXD061384 | Pride | 2025-10-07

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
BM_241129_1056_Lumos_01.raw Raw
BM_241129_1056_Lumos_02.raw Raw
BM_241129_1056_Lumos_03.raw Raw
BM_241129_1056_Lumos_04.raw Raw
BM_241129_1056_Lumos_05.raw Raw
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