IGFBP2 Mediates Human iPSC-Cardiomyocyte Proliferation in a Cellular Contact-Dependent Manner

ABSTRACT: Inducing cardiomyocyte proliferation in situ presents a promising approach for cardiac regeneration following myocardial injury; however, mature cardiomyocytes exhibit inhibitory mechanisms that suppress proliferative signaling. This study investigates the role of cell-cell contact as a key regulator of cardiomyocyte proliferation. Using human iPSC-derived cardiomyocytes (hiPSC-CMs), the findings demonstrate that proliferation initially increases with cell density but is markedly suppressed upon the establishment of intercellular contacts. Phosphoproteomic and phenotypic analyses reveal that cell-cell contact promotes adherens junction formation, enhances sarcomere organization, and increases contractility, collectively contributing to the suppression of proliferation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell, Stem Cell, Cardiac Muscle Cell

SUBMITTER: Sean Humphrey  

LAB HEAD: Sean Humphrey

PROVIDER: PXD061405 | Pride | 2025-12-15

REPOSITORIES: Pride