Dataset Information

The urinary proteome differs with the presence and type of breast cancer

ABSTRACT: Despite the advancements in screening and treatment, the incidence of breast cancer (BC) and associated mortalityare projected to increase. Therefore, developing a companion diagnostic for BC remains important. To explore the urinary proteome for potential biomarkers for BC.  A total of 130 participants were recruited for this study which categorised into four groups; (1) newly diagnosed breast cancer (BC) n=46, (2) benign breast disease (BBD) n=36, (3) symptom control (SC) n=30 and (4) healthy control (HC) n=18. The BC class sub-divided in pre-invasive: ductal carcinoma in situ (DCIS) (n=3), invasive ductal carcinoma (IDC) (n=23) and IDC accompanied DCIS (n=8) classes. Purified urinary proteins were analysed using a bottom-up data dependent acquisition proteomics approach. Protein identifications and abundances were generated using Thermo Scientific TM Proteome Discoverer TM with statistical analyses were performed via open-source software platforms and databases; MetaboAnalyst v6.0, DAVID and STRING v12.0.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

DISEASE(S): Breast Cancer

SUBMITTER: Nur Aimi Aliah Zainurin

LAB HEAD: Luis Mur

PROVIDER: PXD061502 | Pride | 2025-12-15

REPOSITORIES: Pride

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Publications

The Urinary Proteome Differs with the Presence and Type of Breast Cancer.

Zainurin Nur Aimi Aliah NAA Morphew Russell M RM Ganti Alekhya A Ivanova Dimitra D Gate Tim T Tench Helen H Phillips Helen H Pennick Mandana M Mur Luis A J LAJ

Journal of proteome research 20251103 12

Despite advancements in screening and treatment, the incidence of breast cancer (BC) and associated mortality are projected to increase. Therefore, developing a companion diagnostic for BC remains important. Herein, we explore the urinary proteome for biomarkers of BC: 130 urine samples from (1) newly diagnosed breast cancer (BC), n = 46, (2) benign breast disease (BBD), n = 36, (3) symptom control (SC), n = 30, and (4) healthy control (HC), n = 18. The BC class inclu ...[more]

PMID: 41181894

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Project description:The subpopulations of mesenchymal stem cells isolated from breast adipose tissue which display migrated towards conditioned media from MDA-MB231 cell line were expanded for miRNA analysis. The tumor microenvironment (TM) is known to promote tumor growth and progression. Ubiquitously distributed tissue resident stem cells (MSCs) elicit regenerative properties. In addition, they are capable of homing to sites of inflammation, injury, and tumor. Considering the tumor tropic property of MSCs, the interaction between the breast cancer (BC) microenvironment and breast resident adipose tissue derived MSCs (ASCs) merits further investigations. Initial data indicate that a subset of ASCs derived from breast adipose tissue (B-ASCs) display a high affinity towards the conditioned media (CM) from two breast cancer cell lines, MDA-MB231 (MDA-CM) and MCF7 (MCF-CM). Profiling secreted cytokines of these CMs identified significant expression of angiogenin, GM-CSF, and IL-6. While the expression of GRO-M-NM-1, MCP-1, RANTES, and IL-1M-NM-1 is more pronounced in MDA-CM, MCF-CM contains higher amounts of IGFBP2, TRAIL, and ErbB3. Gene expression profiling suggests that despite the distinct differences, both migratory subset of B-ASCs towards MDA-CM and MCF-CM display perturbed expression of genes like KISS1, TNSF1, IL18 and MMP2, which could be associated with a defensive role of B-ASCs. In addition, the BC microenvironment alters microRNA (miRNA) expressions in B-ASCs. in this study the migratory cells were evaluated for miRNA expression versus non-migratory counterparts. as controls unexposed parental cell lines (2) were used on the same hybridization chip in which one labeled Hy3 and other labeled Hy5. The ratio of the control parental cells was used as base nanalysis of miRNA expression in MSCs. we also include another control in this study, the migratory subpopulations of MSCs which display migratory potentials against protein gradient (M5) were analyzed for miRNA expression versus non-migratory counterparts (NM-5). using this control facilitate identification of those miRNA responsive to tumor CM. the data analysis confirm that altered gene and miRNA profiles resulted from exposure of MCF-CM and MDA-CM on B-ASCs are similar to those observed in B-ASCs isolated from breast adipose tissue of BC patients. Analysis the signaling between the B-ASCs and TM may help in understanding the possible role of B-ASCs in stasis, progression, or regression of the BC. The microRNA expression of migratory subpopulations were compared to parental populations of MSCs.

			Action	DRS
	260424_Breast_Cancer_Urine.msf	Msf
	P100_068YGC_BBD.raw	Raw
	P101_069YGC_BBD.raw	Raw
	P102_072YGC_BBD.raw	Raw
	P103_073YGC_BBD.raw	Raw