Proteomics

Dataset Information

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Proteomics of a protein phenol phase sample of human CD138+ cells after RNA extraction , LC-MSMS

ABSTRACT: This study evaluates the performance of adapted SPEED (Sample Preparation by Easy Extraction and Digestion) protocols, a simplified, detergent-free approach tailored for various biological matrices, including lysis-resistant samples. All datasets can be found searching for the keyword NKUAProt001.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bone Marrow

DISEASE(S): Multiple Myeloma

SUBMITTER: Guillaume MĂ©dard  

LAB HEAD: Guillaume MĂ©dard

PROVIDER: PXD061575 | Pride | 2025-11-10

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
P2023-11_Core_ELMUMY_TriTFA_MM_1153_4000cells_1_Slot2-19_1_2865.d.zip Other
P2023-11_Core_ELMUMY_TriTFA_MM_1153_4000cells_2_Slot2-20_1_2867.d.zip Other
P2023-11_Core_ELMUMY_TriTFA_MM_1153_4000cells_3_Slot2-21_1_2869.d.zip Other
experiment_annotation_metadata.tsv Tabular
fragger_dda.params Other
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Publications

Easy Proteomics Sample Preparation: Technical Repeatability and Workflow Optimization Across 8 Biological Matrices in a New Core Facility Setting.

Karousi Paraskevi P   Voumvouraki Maria M   Nikolaou Panagiota Efstathia PE   Kollias Ioannis I   Paradeisi Foteini F   Sampanai Elena E   Gkalea Vasiliki V   Morianos Ioannis I   Zoidakis Jerome J   Kastritis Efstathios E   Thomaidis Nikolaos N   MĂ©dard Guillaume G   Courraud Julie J  

Proteomics 20251024 20

Bottom-up proteomics relies on efficient and repeatable sample preparation for accurate protein identification and precise quantification. This study evaluates the performance of adapted SPEED (Sample Preparation by Easy Extraction and Digestion) protocol, a simplified, detergent-free approach tailored for various biological matrices, including lysis-resistant samples. Protein extraction and denaturation steps were refined for 8 biological matrices enabling standardized, cheap, and scalable prot  ...[more]

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