Proteomics

Dataset Information

Deep phenotyping of SH-Sy5y cells exposed to glucosylsphingosine by LC-MS

ABSTRACT: SH-Sy5y cells were exposed to glucosylsphingosine (GlcSph) at two concentrations observed in moderate (20 ng/mL) and severe (200 ng/mL) Gaucher disease phenotypes and subjected to label free proteomics to identify cellular pathways and molecules affected by GlcSph. Lyso-Gb3, a lipid accumulating in Fabry disease, which exhibits no nerodegeneration, was used as a disease control. DMSO was used as a vehicle control.Proteomics analysis demonstrated a negative effect of GlcSph on cell metabolism, particularly affecting the TCA cycle, mitochondrial function, glycolysis and protein ubiquitination.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Valeria Nikolaenko  

LAB HEAD: Wendy Heywood

PROVIDER: PXD061666 | Pride | 2025-09-01

REPOSITORIES: Pride

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Action DRS
20190907_Fr1_Ctrl_rep_1.raw.tar Raw
20190907_Fr1_Ctrl_rep_2.raw.tar Raw
20190907_Fr1_GlcSph_200_rep_1.raw.tar Raw
20190907_Fr1_GlcSph_200_rep_2.raw.tar Raw
20190907_Fr1_GlcSph_20_rep_1.raw.tar Raw
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