Dataset Information

Pulldown proteomics of XybR in SirexAA-E

ABSTRACT: Streptomyces sp. SirexAA-E chnages secretion of enzymes that can degrade xylan. These enzymes transcription is regulated by a transcriptional regulator. To determine the dominant regulator, we performed pulldown proteomics, using some promoter regions (P0265, P0357, P5230) which are related to xylan catabolism of S. SirexAA-E. Intracellular proteomes were from glucose-grown cells and xylan-grown cells.

INSTRUMENT(S):

ORGANISM(S): Streptomyces Sp. Sirexaa-e

TISSUE(S): Whole Body

SUBMITTER: Taichi E. Takasuka

LAB HEAD: Taichi E. Takasuka

PROVIDER: PXD061680 | Pride | 2025-11-11

REPOSITORIES: Pride

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Source:

			Action	DRS
	061119_Kei_XybR_0265_1.msf	Msf
	061119_Kei_XybR_0265_1.raw	Raw
	061119_Kei_XybR_0265_2.raw	Raw
	061119_Kei_XybR_0265_3.raw	Raw
	061119_Kei_XybR_0357_1.msf	Msf

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Project description:Abstract Background The extraordinarily resistant bacterium Deinococcus radiodurans withstands harsh environmental conditions present in outer space. Deinococcus radiodurans was exposed for one year outside the International Space Station within Tanpopo orbital mission to investigate microbial survival and space travel. In addition, a ground-based simulation experiment with conditions, mirroring those from Low Earth orbit, was performed. Methods We monitored Deinococcus radiodurans cells during early stage of recovery after Low Earth orbit exposure using electron microscopy tools. Furthermore, proteomic, transcriptomic and metabolomic analyses were performed to identify molecular mechanisms responsible for the survival of Deinococcus radiodurans in Low Earth orbit. Results D. radiodurans cells exposed to low Earth orbit conditions do not exhibit any morphological damage. However, an accumulation of numerous outer-membrane associated vesicles was observed. On levels of proteins and transcripts, a multifaceted response was detected to alleviate cell stress. The UvrABC endonuclease excision repair mechanism was triggered to cope with DNA damage. Defense against reactive oxygen species is mirrored by the increased abundance of catalases and is accompanied by the increased abundance of putrescine which works as scavenging molecule. In addition, several proteins and mRNAs, responsible for regulatory and transporting functions showed increased abundances. The decrease in primary metabolites indicate alternations in the energy status, which is needed to repair damaged molecules. Conclusion Low Earth orbit induced molecular rearrangements trigger multiple components of metabolic stress response and regulatory networks in exposed microbial cells. Presented results show that the non-sporulating bacterium Deinococcus radiodurans survived long-term Low Earth orbit exposure if wavelength below 200 nm are not present, which mirrors the UV spectrum of Mars, where CO2 effectively provides a shield below 190 nm. These results should be considered in the context of planetary protection concerns and the development of new sterilization techniques for future space missions.

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Pulldown proteomics of XybR in SirexAA-E

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