Proteomics

Dataset Information

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Identification of Sh3rf3-interacting proteins in mouse prefrontal cortex by immunoprecipitation mass spectrometry

ABSTRACT: Sh3rf3 is an ASD-associated gene,Sh3rf3 deficiency reduces excitatory synaptic strength in prefrontal cortex and decreases the readily releasable vesicle pool and attenuated vesicle replenishment.To delve into the underlying mechanisms for impaired excitatory transmission, we performed immunoprecipitation mass spectrometry (IP-MS) in Sh3rf3-3×flag knock-in mice we generated. Gene Ontology analysis of proteins that interacted with SH3RF3 indicated that they were highly associated with synaptic transmission and vesicle recycling.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Cns Neuron (sensu Vertebrata)

DISEASE(S): Autism Spectrum Disorder

SUBMITTER: Yuting Yuan  

LAB HEAD: Zhiheng Xu

PROVIDER: PXD061685 | Pride | 2026-03-23

REPOSITORIES: Pride

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Dataset's files

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ASD_Mouse_PFC_FLAG_Rep1_IP-MS.raw Raw
ASD_Mouse_PFC_FLAG_Rep1_IP-MS.xlsx Xlsx
ASD_Mouse_PFC_FLAG_Rep2_IP-MS.raw Raw
ASD_Mouse_PFC_FLAG_Rep2_IP-MS.xlsx Xlsx
ASD_Mouse_PFC_FLAG_Rep3_IP-MS.raw Raw
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Publications

Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice.

Yuan Yuting Y   Li Yang Y   Yang Fuqiang F   Jiang Yisheng Y   Ding Yuanyuan Y   Xiao Ying Y   Zhu Xili X   Shu Xingmei X   Huang Xiahe X   Wang Yingchun Y   Zhang Shuli S   Sun Jianyuan J   Xu Zhiheng Z  

Molecular psychiatry 20251125

Autism spectrum disorder (ASD) is a prevalent and complex neurodevelopmental disorder with a strong genetic basis. Although SH3RF3 has been identified as an ASD candidate gene, its biological function and pathophysiological mechanisms remain elusive. Here, we reveal that SH3RF3 functions as an essential scaffold protein that facilitates presynaptic vesicle docking. Mechanistically, it orchestrates the formation of a molecular complex between the kinase BRSK1/SAD-B and the ASD-associated active z  ...[more]

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