Proteomics

Dataset Information

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Protein interactome of HNRNPU in differentiating neural cells

ABSTRACT: HNRNPU is an RNA-binding protein with diverse roles in regulating gene expression. Pathogenic genetic variants of HNRNPU cause a severe neurodevelopmental disorder (NDD), but the underlying molecular mechanisms are unclear. Here, we investigated the protein-protein interaction (PPI) network of HNRNPU in neuroepithelial stem cells (NES) and differentiating neural cells derived from human induced pluripotent stem cells. Using high-throughput approaches, we found HNRNPU interacting with the mammalian SWI/SNF complex and highlight its putative role in multiple stages of mRNA regulation. Notably, both PPI partners and interacting mRNAs implicated HNRNPU in translation, a role that had not been recognized before.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Neuron Differentiation, Neuronal Stem Cell

SUBMITTER: Marika Oksanen  

LAB HEAD: Kristiina Tammimies

PROVIDER: PXD061718 | Pride | 2026-02-23

REPOSITORIES: Pride

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Action DRS
IP-MS_D0_IP_1.raw Raw
IP-MS_D0_IP_2.raw Raw
IP-MS_D0_IP_3.raw Raw
IP-MS_D0_IgG_1.raw Raw
IP-MS_D0_IgG_2.raw Raw
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Publications

Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation.

Oksanen Marika M   Mastropasqua Francesca F   Mazan-Mamczarz Krystyna K   Martindale Jennifer L JL   Ye Xuan X   Arora Abishek A   Banskota Nirad N   Gorospe Myriam M   Tammimies Kristiina K  

Nucleic acids research 20260201 4

HNRNPU is an RNA-binding protein with diverse roles in transcriptional and post-transcriptional regulation. Pathogenic genetic variants of HNRNPU cause a severe neurodevelopmental disorder (NDD), but the underlying molecular mechanisms are unclear. Here, we comprehensively investigate the HNRNPU molecular interactome by integrating protein-protein interaction (PPI) mapping, RNA target identification, and genome-wide DNA methylation profiling in human neuroepithelial stem cells and differentiatin  ...[more]

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