Proteomics

Dataset Information

0

Analysis of mini-SHIP1 with pY Peptide by HDX-MS

ABSTRACT: Hydrogen deuterium exchange mass spectrometry (HDX-MS) was used to analyze how the mini-SHIP1 construct interacts with receptor-derived phosphotryrosine peptides (pY) to probe how these peptides regulate SHIP1 autoinhibition. This HDX-MS helped to identify intramolecular contacts involved in the regulation of SHIP1 autoinibition. Results from this HDX analyzing the monomeric mini-SHIP1 construct are used in tandem with HDX-MS results analyzing the a dimeric SHIP1 construct to confirm the same mechanism of autoinhibtion exists. These results help confirm autoinhibition of monomeric SHIP1 is indeed likely regulated by the same mechanism to that of dimeric SHIP1.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: John Burke

PROVIDER: PXD061719 | Pride | 2025-10-15

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
06_26_23_MPHDX36_P_300s_1_0_01_22434.d.zip Other
06_26_23_MPHDX36_P_3s_1_0_01_22432.d.zip Other
06_26_23_MPHDX36_S_300s_1_0_01_22433.d.zip Other
06_26_23_MPHDX36_S_3s_1_0_01_22430.d.zip Other
06_26_23_MPHDX36_S_3s_2_0_01_22435.d.zip Other
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Publications

SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase.

Drew Emma E EE   Nyvall Hunter G HG   Parson Matthew A H MAH   Talus Reed K RK   Burke John E JE   Hansen Scott D SD  

The Journal of biological chemistry 20251006

The Src homology 2 (SH2) domain containing inositol polyphosphate 5-phosphatase 1 (SHIP1) is an immune cell specific enzyme that regulates phosphatidylinositol-(3,4,5)-trisphosphate signaling at the plasma membrane following receptor activation. SHIP1 plays an important role in processes such as directed cell migration, endocytosis, and cortical membrane oscillations. Alterations in SHIP1 expression have been shown to perturb myeloid cell chemotaxis and differentiation. In the brain, SHIP1 regul  ...[more]

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