Dataset Information

Changes in the Proteome and Phosphoproteome of Zea mays Tissues in Drought Stress Show Plant Tissue Re-sponses from Dehydrins, Carboxylic Acid Metabolism, RNA Splicing and Transcription Factors

ABSTRACT: Background: Maize is a vital crop, supporting 19.5% of global calorie intake. However, maize is vulnerable to even brief periods of drought which substantially re-duces seed set and therefore yield. Methods: To identify proteins involved in responses of maize to drought, soluble proteins were extracted from leaf and silk tissues of Zea mays and protein abundance and phosphorylation status were quantified relative to well-watered controls. Label-free quantification and phosphopeptide enrichment were applied to the same biological samples and over 300 proteins were identified with sig-nificantly different changes. Results: Proteins known to be involved in drought responses were identified, such as the abscisic acid pathway and transcription factors. Of particular interest is a group of dehydrins quantified at both total protein and phosphopeptide levels, permitting insight into stoichiometry. The biological function of dehydrins in the model plant Arabidopsis thaliana is known to be regulated by phosphorylation. Conclu-sions: Translation of protein function from model plant to crops remains highly chal-lenging because genome duplication has created complex sets of orthologous and ho-mologous proteins. By focusing on proteomic changes during crop stress responses, this work enables the identification of known and novel proteins, substantially aiding the transfer of knowledge from model plants to crops.

INSTRUMENT(S):

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Leaf, Corn Silk

DISEASE(S): Disease Free

SUBMITTER: Georgina Charlton

LAB HEAD: Alex Jones

PROVIDER: PXD061787 | Pride | 2026-05-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Maize_Leaf_LFQ_txt.7z	Other
	Maize_Leaf_Phos_txt.7z	Other
	Maize_Silk_LFQ_txt.7z	Other
	Maize_Silk_Phos_txt.7z	Other
	OT_190708_24_MS19-164_Leaf_C4.raw	Raw

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Dataset Information

Changes in the Proteome and Phosphoproteome of Zea mays Tissues in Drought Stress Show Plant Tissue Re-sponses from Dehydrins, Carboxylic Acid Metabolism, RNA Splicing and Transcription Factors

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