Proteomics

Dataset Information

0

Identification of p62-interacting proteins by mass spectrometry analysis in HEK293T cells


ABSTRACT: HEK293T cells were ectopically expressing Flag tagged p62 (p62 group) or empty vectors (Control group) for 48h, cell lysates were incubated with anti-Flag affinity gels, and co-immunoprecipitates were subjected to trypsin digestion followed by mass spectrometry analysis. Peptides were separated by the EASY-nLC system (Thermo Fisher) and analyzed by the Q Exactive mass spectrometer (Thermo Fisher). Protein analysis were performed with Thermo Proteome Discoverer 2.1 (Thermo Fisher) and searched against Uniprot Human database. Three samples per group.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: yun yang  

LAB HEAD: yang yun

PROVIDER: PXD061794 | Pride | 2025-07-29

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
YS_CHI_194_P62_1.raw Raw
YS_CHI_194_P62_2.raw Raw
YS_CHI_194_P62_3.raw Raw
YS_CHI_194_V_1.raw Raw
YS_CHI_194_V_2.raw Raw
Items per page:
1 - 5 of 8
altmetric image

Publications

TRIM25 ubiquitinates and degrades p62/SQSTM1 to suppress autophagy.

Qiu Xiang X   Ren Jin J   Yang Yun Y   Hu Weikang W   Wang Chengcheng C   Hu Ronggui R   Li Chuanyin C  

Acta biochimica et biophysica Sinica 20250620


Similar Datasets

2025-07-29 | PXD062511 | Pride
2018-03-31 | E-MTAB-4894 | biostudies-arrayexpress
2014-10-10 | E-GEOD-62210 | biostudies-arrayexpress
2014-07-23 | E-GEOD-55587 | biostudies-arrayexpress
2022-03-06 | GSE197894 | GEO
2023-12-31 | GSE196019 | GEO
2023-05-30 | GSE229713 | GEO
2021-06-07 | GSE173094 | GEO
2019-08-23 | GSE119042 | GEO
2019-08-23 | GSE119041 | GEO