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GlycoFASP: A Universal Method to Prepare Complex Mixtures for O-Glycoproteomic Analysis

ABSTRACT: Here we describe a method titled filter-aided preparation of O-glycoproteomic samples (GlycoFASP) which takes advantage of molecular-weight cut-off filters and O-glycoprotease digestion specificity. Using GlycoFASP, preparation for MS analysis of biological samples is completed within one day and only requires one milligram of sample. It is both low-cost and amenable to non-specialists. GlycoFASP regularly achieves depletion of nearly all non-O-glycosylated proteins, with ≥70% of the protein signal originating from O-glycoproteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Semen, Blood Serum

SUBMITTER: Shane Finn

LAB HEAD: Stacy Malaker

PROVIDER: PXD061804 | Pride | 2026-07-08

REPOSITORIES: Pride

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			Action	DRS
	07-27_Amicon_10_kDa.xlsx	Xlsx
	07-27_Microcon_10_kDa.xlsx	Xlsx
	07-27_Pierce_10_kDa.xlsx	Xlsx
	07-27_Vivacon_10_kDa.xlsx	Xlsx
	08-06_Filter_10_kDa_1.xlsx	Xlsx

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Publications

GlycoFASP: A Universal Method to Prepare Complex Mixtures for O-Glycoproteomic Analysis.

Finn Shane M SM Mahoney Keira E KE Lucas Taryn M TM Rangel-Angarita Valentina V Chen Ryan J RJ Malaker Stacy A SA

Analytical chemistry 20251022 43

Glycoproteomic analyses remain a significant challenge due to the considerable heterogeneity in the composition, linkage, and localization of glycosylation. These issues are further complicated in the study of O-glycosylation, which primarily occurs on serine or threonine residues, given the lack of a consensus peptide motif and the proximity of O-glycosites. Mass spectrometry (MS)-based analysis is the premier technique for analyzing O-glycoproteins,but often equires prior ...[more]

PMID: 41124113

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