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Silencing mitochondrial gene expression in living cells – CYTB- and ND2-KD data

ABSTRACT: Mitochondria fullfill central functions in cellular metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms underlying mitochondrial gene expression remain enigmatic. A lack of suitable technologies to target mitochondrial protein synthesis in living cells has limited experimental access. Here, we silence the translation of specific mitochondrial mRNAs in living cells by delivering synthetic peptide-morpholino chimeras. This approach allows comprehensive temporal monitoring of cellular responses. Our study provides insights into mitochondrial translation, its integration into cellular physiology, and represents an innovative strategy to address mitochondrial gene expression in living cells. The tools that we present can readily be applied to analyze the mechanisms and pathophysiology of mitochondrial gene expression in a broad range of cellular model systems.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Julian Bender

LAB HEAD: Bettina Warscheid

PROVIDER: PXD061846 | Pride | 2025-04-17

REPOSITORIES: Pride

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			Action	DRS
	MaxQ_txt_CYTB_ND2-KDs.zip	Other
	QEplus043283.raw	Raw
	QEplus043284.raw	Raw
	QEplus043285.raw	Raw
	QEplus043286.raw	Raw

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