Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mouse KPC cell secreted extracellular vesicle LC-MSMS

ABSTRACT: For Mouse KPC cell secreted extracellular vesicle LC-MSMS, extracellular vesicles in Alkbh5-WT or Alkbh5-KO KPC cell culture medium were isolated by ultracentrifugation. The protein and its levels in extracellular vesicle were determined through LC-MSMS.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell

SUBMITTER: Claire Chow

LAB HEAD: Claire Chow

PROVIDER: PXD061967 | Pride | 2025-03-26

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	KPC-A5KO-EV1_Slot1-36_1_5753.d.zip	Other
	KPC-A5KO-EV2_Slot1-37_1_5754.d.zip	Other
	KPC-NC-EV1_Slot1-31_1_5748.d.zip	Other
	KPC-NC-EV2_Slot1-34_1_5751.d.zip	Other
	Maxquant_Result.zip	Other

Items per page:

1 - 5 of 6

Similar Datasets

Mouse pancreatic cancer cells LC-MSMS

Project description:For liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of mouse pancreatic cancer cells, we injected green fluorescent protein (GFP)-labeled KPC cells into the pancreases of C57BL/6J mice to establish orthotopic pancreatic cancer models. Following stress treatment, we isolated the GFP-labeled pancreatic cancer cells from the allografts of the stress-treated or control C57BL/6J mice. Subsequently, we conducted protein mass spectrometry on these GFP-labeled tumor cells.

2025-03-26 | PXD061963 | Pride

Caenorhabditis elegans extracellular vesicle

Project description:Caenorhabditis elegans extracellular vesicle

| PRJNA612535 | ENA

Project description:Plasma microbial extracellular vesicle

| PRJNA796437 | ENA

Project description:small extracellular vesicle microRNA sequence

| PRJNA657371 | ENA

Analysis of small extracellular vesicle derived miRNAs using high through-put miRNA sequencing

Project description:To gain insight into the microRNA expression profile of small extracellular vesicle derived from different cell types and to verify their mechanism, we utilized the miRNA sequencing technology to analyze the miRNA profiles of different human cell derived small extracellular vesicle

2024-05-01 | GSE203445 | GEO

Extracellular vesicle labeled Granulocyte macrophage progenitors

Project description:Extracellular vesicle labeled Granulocyte macrophage progenitors

| PRJNA525660 | ENA

Extracellular Vesicle P100 Fraction Small RNAs

Project description:Extracellular Vesicle P100 Fraction Small RNAs

| PRJNA498024 | ENA

BAG6KO impacts extracellular vesicle mRNA cargo

Project description:BAG6KO impacts extracellular vesicle mRNA cargo

| PRJEB28086 | ENA

Identification of extracellular vesicle miRNA cargo

Project description:Identification of extracellular vesicle miRNA cargo

| PRJNA601108 | ENA

ALKBH5 is a New Mammalian RNA Demethylase

Project description:N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells. RNA-seq in two cell types

2013-02-15 | E-GEOD-40132 | biostudies-arrayexpress