Dataset Information

Qualitative proteomics of Priestia megaterium

ABSTRACT: Polyhydroxyalkanoates (PHAs) production using cellulosic biomass is a promising way for sustainable manufacturing of bioplastics. The famous bacterium, Priestia megaterium, is an ideal choice because it can utilize glucose and xylose for PHA synthesis. In the present study, we engineered the genome of P. megaterium by CRISPR-Cas9 system to enhance cellobiose utilization and PHA production. The genes encoding for β-glucosidases (Bgls) from different microbes were introduced into the P. megaterium to improve the cellobiose utilization.

INSTRUMENT(S):

ORGANISM(S): Priestia Megaterium Nbrc 15308 = Atcc 14581

TISSUE(S): Whole Body

SUBMITTER: Taichi E. Takasuka

LAB HEAD: Taichi E. Takasuka

PROVIDER: PXD062017 | Pride | 2026-04-03

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 9

Similar Datasets

Project description:Cells must sense and respond to available nutrients to utilize available resources and establish fungal colonies. Saprophytic microbes harvest carbon from plant biomass, which contain insoluble, complex carbohydrates that must be degraded extracellularly prior to import into the cell. Filamentous fungi can degrade these complex carbohydrates. However, while many single cellular microbes can utilize the building blocks of these insoluble polysaccharides, they frequently are unable to degrade insoluble carbohydrates. Cellobiose is a disaccharide breakdown product of cellulose, the most abundant component of plant biomass. We investigated the genetic regulation of cellobiose utilization in the oleaginous, basidiomycete yeast Rhodotorula (Rhodosporidium) toruloides using a combination of phylogenetic, genetic, and genomic approaches. We tested the closest R. toruloides homolog of the cellulose degradation regulator CLR-2/ClrB for a role in cellobiose utilization. Deletion of this transcription factor, which we named Cbr1, eliminated the ability of cells to grow on media containing cellobiose as the sole carbon source. Although, the role of CLR-2/ClrB is limited to regulating the expression of genes involved in the utilization of cellulose and hemicellulose, Cbr1 plays a role not only in cellobiose utilization, but also utilization of tricarboxylic acid (TCA) cycle intermediates. Transcriptional profiling revealed that the genes activated by Cbr1 included genes encoding beta-glucosidases and transporters. These beta-glucosidases are secreted and are necessary and sufficient for cellobiose utilization, while one of the transporters is required for utilization of the TCA cycle intermediate, citrate. Additionally, Cbr1 inhibits carbon catabolite repression, specifically during utilization of disaccharides, which may have evolved to limit the repression of genes encoding secreted proteins that cleave disaccharides into glucose extracellularly. The co-regulation of cellobiose and TCA cycle utilization with carbon catabolite repression may shed light on alternative methods of the regulation of carbon catabolite repression in fungi.

			Action	DRS
	GH1_Bs.msf	Msf
	GH1_Bs.raw	Raw
	GH1_Ss.msf	Msf
	GH1_Ss.raw	Raw
	GH1_Ta.msf	Msf

Dataset Information

Qualitative proteomics of Priestia megaterium

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets